Strategies for interfacing solid-phase microextraction with liquid chromatography.
Strategies for interfacing solid-phase microextraction with liquid chromatography.
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DOI：
10.1016/j.chroma.2006.11.073
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发表时间：
2007-06
期刊：
影响因子：
--
通讯作者：
H. Lord
中科院分区：
文献类型：
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作者：
H. Lord
固相微萃取(SPME)技术同样适用于挥发性和非挥发性分析物，但在应用于气相分离方面的进展超过了液相分离。固相微萃取与气相色谱设备的接口一直很简单，几乎不需要对现有设备进行修改。然而，非挥发性或热不稳定分析物的溶剂解吸要求对于固相微萃取与液相分离的接口已被证明是具有挑战性的。在过去的十年里，文献中描述了许多实现这一目标的选择，并在几个不同的分析领域中进行了应用。到目前为止，还没有一个单一的策略或接口设备设计被证明是最佳的。在方法开发过程中，分析人员必须从可用选项中选择最合适的接口技术。在这些选择中，出现了三种一般策略：(1)使用手动进样接口TEE；(2)管内固相微萃取；(3)离线解吸，然后进行常规液体进样。此外，人们还有兴趣将SPME直接与电喷雾电离和基质辅助激光解吸电离(MALDI)联用，以用于质谱学。这里回顾了这些策略的几个例子，并对它们的使用和应用进行了概述。
Solid-phase microextraction (SPME) techniques are equally applicable to both volatile and non-volatile analytes, but the progress in applications to gas-phase separations has outpaced that of liquid-phase separations. The interfacing of SPME to gas chromatographic equipment has been straight-forward, requiring little modification of existing equipment. The requirement of solvent desorption for non-volatile or thermally labile analytes has, however, proven challenging for interfacing SPME with liquid-phase separations. Numerous options to achieve this have been described in the literature over the past decade, with applications in several different areas of analysis. To date, no single strategy or interface device design has proven optimal. During method development analysts must select the most appropriate interfacing technique among the options available. Out of these options three general strategies have emerged: (1) use of a manual injection interface tee; (2) in-tube SPME; and (3) off-line desorption followed by conventional liquid injection. In addition, there has been interest in coupling SPME directly to electrospray ionisation and matrix-assisted laser desorption ionisation (MALDI) for mass spectrometry. Several examples of each of these strategies are reviewed here, and an overview of their use and application is presented.