Genome analysis provides insights into the biocontrol ability of Mitsuaria sp. strain TWR114
Genome analysis provides insights into the biocontrol ability of Mitsuaria sp. strain TWR114
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DOI：
10.1007/s00203-021-02327-1
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发表时间：
2021-04
影响因子：
2.8
通讯作者：
M. Marian;T. Fujikawa;M. Shimizu
中科院分区：
文献类型：
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作者：
M. Marian;T. Fujikawa;M. Shimizu
密苏利亚属TWR114 是一种针对番茄青枯病 (TBW) 的生物防治剂。我们的目的是获得与该菌株的生物防治机制和定殖能力相关的基因组见解。基因组草案大小为 5,632,523 bp，GC 含量为 69.5%，组装成 1144 个支架。基因组注释总共预测了 4675 个蛋白质编码序列 (CDS)、914 个假基因、49 个转移 RNA、3 个非编码 RNA 和 2 个核糖体 RNA。基因组分析确定了与氨基酸和碳水化合物的代谢和运输、运动和趋化能力、应激保护（氧化、抗生素和噬菌体）、次级代谢产物、肽酶、群体淬灭酶和吲哚-3-乙酸的产生以及蛋白质分泌系统及其相关附属物的各种途径相关的多个CDS。该基因组资源将加深我们对与 TWR114 生物防治和定殖能力相关的基因组特征的理解，并促进其作为抗 TBW 的新型生物农药的开发。
Mitsuariasp. TWR114 is a biocontrol agent against tomato bacterial wilt (TBW). We aimed to gain genomic insights relevant to the biocontrol mechanisms and colonization ability of this strain. The draft genome size was found to be 5,632,523 bp, with a GC content of 69.5%, assembled into 1144 scaffolds. Genome annotation predicted a total of 4675 protein coding sequences (CDSs), 914 pseudogenes, 49 transfer RNAs, 3 noncoding RNAs, and 2 ribosomal RNAs. Genome analysis identified multiple CDSs associated with various pathways for the metabolism and transport of amino acids and carbohydrates, motility and chemotactic capacities, protection against stresses (oxidative, antibiotic, and phage), production of secondary metabolites, peptidases, quorum-quenching enzymes, and indole-3-acetic acid, as well as protein secretion systems and their related appendages. The genome resource will extend our understanding of the genomic features related to TWR114’s biocontrol and colonization abilities and facilitate its development as a new biopesticide against TBW.