SYNTHETIC CHEMORECEPTIVE MEMBRANES - SENSING BITTER OR ODOROUS SUBSTANCES ON A SYNTHETIC LIPID MULTIBILAYER FILM BY USING QUARTZ-CRYSTAL MICROBALANCES AND ELECTRIC RESPONSES
SYNTHETIC CHEMORECEPTIVE MEMBRANES - SENSING BITTER OR ODOROUS SUBSTANCES ON A SYNTHETIC LIPID MULTIBILAYER FILM BY USING QUARTZ-CRYSTAL MICROBALANCES AND ELECTRIC RESPONSES
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DOI：
10.1021/ac00213a017
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发表时间：
1990-07-15
影响因子：
7.4
通讯作者：
EBATO, H
中科院分区：
文献类型：
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作者：
OKAHATA, Y;ENNA, G;EBATO, H
通过观察多层膜石英晶体微天平(QCM)的频率变化，检测了苦味或气味物质在合成脂质多层基质(2C18N+2C1/PSS-)上的特异性吸附。用QCM法可定量测定这些物质在脂质基质中的分配系数(P)和扩散常数(D)。各种苦味或气味物质在QCM上合成的双层膜上的分配系数与人体苦味或嗅觉感受的强度有很好的相关性：苦味或气味物质的强度越强，其在脂质基质上的吸附就越大。这表明，脂质包裹的QCM对苦味和气味具有灵敏和选择性的传感器作用。2C18N+2C1/PSS-膜的电响应(膜电位和膜电阻的变化)是通过对这些物质的吸附而连续发生的。苦味或有气味的物质在较低浓度下表现出较强的诱导膜电位变化。研究发现，具有立体大分子结构的苦味物质吸附在脂质基质的表面，从而改变了膜的相边界电位。相反，具有较小或细长结构的气味物质可以穿透到脂质基质中，导致膜电阻降低(离子通透性增加)。通过对具有不同分子结构的简单C9-10疏水醇类的吸附研究，证实了分子形态对苦味和气味物质的选择性吸附行为。
Specific adsorptions of bitter or odorous substances on a synthetic lipid multibilayer matrix (2C18N+2C1/PSS-) were detected by observing frequency changes of a multibilayer-coated quartz-crystal microbalance (QCM). Partition coefficient (P) and diffusion constants (D) of these substances in the lipid matrix could be obtained quantitatively by using the QCM method. There were good correlations between partition coefficient of various bitter or odor substances to the synthetic multibilayer film on the QCM and the intensity of bitter tastes or olfactory receptions in humans: the stronger the intensity of a bitter substance or odorant, the greater the adsorption on the lipid matrix. This indicates that the lipid-coated QCM acts as a sensitive and selective sensor for bitter taste and odor. Electric responses (changes of membrane potential and membrane resistance) of the 2C18N+2C1/PSS- film occurred consecutive by the adsorption of these substances. The bitter or odor substance showing the stronger intensity induced membrane potential change in lower concentrations. It was found that bitter substances having sterically bulky molecular structures absorb on the surface of the lipid-matrix, and the phase-boundary potential of the membrane is thereby changed. On the contrary, odor substances with relatively small or slender structures can penetrate into the lipid matrix and cause reduction of the membrane resistance (the increase of ion permeability). The selective adsorption behavior of bitter and odor substances by molecular shapes was confirmed by adsorption studies of simple C9-10 hydrophobic alcohols having various molecular structures.