ESTIMATION OF EFFECTIVE DIFFUSION-COEFFICIENTS OF MODEL SOLUTES THROUGH GASTRIC MUCUS - ASSESSMENT OF A DIFFUSION CHAMBER TECHNIQUE BASED ON SPECTROPHOTOMETRIC ANALYSIS
ESTIMATION OF EFFECTIVE DIFFUSION-COEFFICIENTS OF MODEL SOLUTES THROUGH GASTRIC MUCUS - ASSESSMENT OF A DIFFUSION CHAMBER TECHNIQUE BASED ON SPECTROPHOTOMETRIC ANALYSIS
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DOI：
10.1039/an9911601113
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发表时间：
1991-11-01
期刊：
影响因子：
4.2
通讯作者：
VADGAMA, P
中科院分区：
文献类型：
--
作者：
DESAI, MA;VADGAMA, P
报道了一种基于分光光度分析的扩散室技术，用于测定不同相对分子质量(M(R))值和不同性质的溶质通过天然粘液凝胶的有效扩散系数。对于所研究的所有溶质，观察到有效扩散系数的减小，溶质通量的延迟至少为原来的两倍。对于所研究的溶质(M(R)值在126-14400 u之间)，低M(R)值的溶质对粘液的滞留作用没有明显的一致性，但在高M(R)值(>；4000u)时，滞留作用大大增强。观察到低M(R)的带电溶质与延迟程度之间的可能关系，这可能表明溶质与带负电荷较大的粘液凝胶存在离子相互作用。这些结果进一步证明，粘液不仅仅是静止水层的凝胶载体，而且可能是特定溶质的更强大的扩散障碍。
A diffusion chamber technique based on spectrophotometric analysis to determine effective diffusion coefficients for solutes with various relative molecular mass (M(r)) values and properties, passing through native mucus gel, is reported. For all solutes studied, a reduction in effective diffusion coefficients is observed with a retardation of solute flux by a factor of at least two. For the solutes investigated (with M(r) values ranging from 126-14400 u), no consistent effect of solutes of low M(r) was evident with regard to the retarding effect of mucus; however, at high M(r) values (> 4000 u) the retardation was greatly enhanced. A possible relationship between charged solutes of low M(r) and the degree of retardation was observed, which possibly suggests the presence of ionic interactions of the solutes with the largely negatively charged mucus gel. The results provide further evidence for the suggestion that mucus is acting more than simply as a gel support for an unstirred water layer and is perhaps a more potent diffusion barrier to specific solutes.