Matrix assisted laser desorption/ionization of potassium adapted angiotensine II using femtosecond laser pulses
Matrix assisted laser desorption/ionization of potassium adapted angiotensine II using femtosecond laser pulses
复制标题
使用飞秒激光脉冲基质辅助激光解吸/电离钾适应血管紧张素 II
DOI：
10.1140/epjd/e2009-00012-2
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
A. Lindinger
中科院分区：
文献类型：
--
作者：
J. Wichmann;C. Lupulescu;L. Wöste;A. Lindinger
用中心波长为400的飞秒激光脉冲进行基质辅助激光解吸/电离(MALDI)测量。被检查的分析物是钾适应的血管紧张素II，其中基质是芥子酸和α-氰基-4-羟基肉桂酸的组合。为了研究获得的钾适应的血管紧张素II复合体的MALDI过程，进行了脉冲能量依赖的测量。结果表明，钾适应的血管紧张素Ⅱ具有较高的稳定化倾向，可用于将非常脆弱的不稳定大分子带入气相。概述的方法是试图通过关注钾适应的血管紧张素II的质谱图来解释MALDI的潜在过程。
Matrix assisted laser desorption/ionization (MALDI) measurements with femtosecond laser pulses at a central wavelength of 400 are presented. The examined analyte is potassium adapted angiotensine II whereby the matrix is a combination of sinapic acid and α-cyano-4-hydroxycinamic acid. Pulse energy dependent measurements are performed in order to investigate the MALDI processes for the obtained potassium adapted angiotensine II complexes. The results show the higher stabilization tendency of the potassium adapted angiotensine II which could be used to bring very fragile non-stable macromolecules into the gas phase. The outlined approach is an attempt to explain the underlying MALDI process by focusing on the mass spectra of potassium adapted angiotensine II.