Real-time and Label-free Monitoring of Biomolecular Interactions within Complex Biological Media Using a Silica Colloidal Crystal Film.
Real-time and Label-free Monitoring of Biomolecular Interactions within Complex Biological Media Using a Silica Colloidal Crystal Film.
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DOI：
10.1021/acsami.0c10926
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发表时间：
2020-07
影响因子：
9.5
通讯作者：
Qianqian Su;Pengfei Xu;Lele Zhou;Feng Wu;Ao Dong;Yizhen Wan;Weiping Qian
中科院分区：
文献类型：
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作者：
Qianqian Su;Pengfei Xu;Lele Zhou;Feng Wu;Ao Dong;Yizhen Wan;Weiping Qian
描述了一种能够实时和无标记地监测全血内的生物分子相互作用的方法，无需任何样品分离和标记过程。这是使用二氧化硅胶体晶体（SCC）薄膜，三维有序的二氧化硅颗粒阵列，其干涉效应是其光学厚度的函数，作为干涉敏感基板。用光谱法研究了免疫球蛋白G（IgG）与金黄色葡萄球菌蛋白A（SPA）结合物之间的相互作用及其SCC膜光学厚度的变化。在缓冲液和全血中实现了IgG的成功检测。该系统构成了一个简单的无标记分析，在监测复杂的生物介质中的生物分子之间的相互作用显示出巨大的潜力。
A method capable of real-time and label-free monitoring of biomolecular interactions within whole blood, with-out any sample separation and label process is described. This was accomplished using silica colloidal crystal (SCC) films, three-dimensionally ordered silica particle arrays whose interference effect is a function of their optical thickness, as interfer-ence sensitive substrates. Interaction between immunoglobulins G (IgG) and protein A from staphylococcus aureus (SPA) con-jugates with changes in optical thickness of SCC films was monitored spectroscopically. Successful detection of IgG was achieved in buffer and whole blood. This system constitutes a simple label-free analysis showing great potential in monitoring interactions between biomolecules in complex biological media.