Long Non-Coding RNA NEAT1 Serves as Sponge for miR-365a-3p to Promote Gastric Cancer Progression via Regulating ABCC4
Long Non-Coding RNA NEAT1 Serves as Sponge for miR-365a-3p to Promote Gastric Cancer Progression via Regulating ABCC4
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DOI：
10.2147/ott.s245557
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发表时间：
2020-01-01
影响因子：
4
通讯作者：
Chang, Zhiwei
中科院分区：
文献类型：
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作者：
Gao, Ming;Liu, Liying;Chang, Zhiwei
前言：据报道，长链非编码RNA（lncRNA）是癌症的关键调节因子。本研究旨在探讨核旁斑装配转录本1（NEAT 1）在胃癌中的生物学作用。方法：采用实时荧光定量聚合酶链反应（qRT-PCR）技术检测NEAT 1在胃癌细胞和正常细胞中的表达。通过Cell Counting Kit-8分析、集落形成分析、伤口愈合分析和流式细胞术分析NEAT 1过表达或下调后的GC细胞行为。应用生物信息学工具分析NEAT 1在胃癌中的意义。结果：NEAT 1在胃癌组织和胃癌细胞中的表达增加，与胃癌患者的总体生存率相关。我们发现NEAT 1过表达促进，而其敲低抑制体外GC细胞增殖，集落形成，侵袭和细胞周期进展。结论：NEAT 1/miR-365 a-3 p/ABCC 4三联体在胃癌的发生发展中起重要作用，可能为胃癌的靶向治疗提供新的靶向标志物。
Introduction: Long non-coding RNA (lncRNA) was reported to be a crucial regulator in cancer. In this work, our purpose is to explore the biological roles of nuclear paraspeckle assembly transcript 1 (NEAT1) in gastric cancer (GC).Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect NEAT1 expression in GC cells and normal cells. GC cell behaviors after NEAT1 overexpression or downregulation were analyzed by Cell Counting Kit-8 assay, colony formation assay, wound-healing assay, and flow cytometry assay. Bioinformatic tools were used to analyze the significance of NEAT1 in GC. The involvement of microRNA-365a-3p (miR-365a-3p) and ATP-binding cassette subfamily C member 4 (ABCC4) in the biological roles of NEAT1 in GC progression was validated by luciferase activity reporter assay and rescue experiments.Results: We found NEAT1 increased expression in both GC tissues and cells and correlated with poorer overall survival of cancer patients. We found NEAT1 overexpression promotes, while its knockdown inhibits GC cell proliferation, colony formation, invasion, and cell cycle progression in vitro. Mechanism analyses showed that NEAT1 serves as a ceRNA to upregulate ABCC4 expression via sponging miR-365a-3p.Conclusion: In this study, we revealed a NEAT1/miR-365a-3p/ABCC4 triplet in GC progression, which may provide novel targeted therapy markers for GC.