Non-specific adhesion on biomaterial surfaces driven by small amounts of protein adsorption
Non-specific adhesion on biomaterial surfaces driven by small amounts of protein adsorption
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DOI：
10.1016/j.colsurfb.2009.05.028
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发表时间：
2009-10-15
影响因子：
5.8
通讯作者：
Santore, Maria M.
中科院分区：
文献类型：
--
作者：
Kalasin, Surachate;Santore, Maria M.
这项工作探讨了由少量吸附的纤维蛋白原引起的长程非特异性相互作用如何潜在地影响生物粘附。生物材料上吸附的蛋白质与接近的细胞或细菌之间的这种非特异性相互作用可能补充甚至主导配体-受体交配。这项工作考虑了生物材料表面和接近的模型细胞（微米级二氧化硅颗粒）表现出强静电排斥的情况，就像诊断和芯片实验室应用中的情况一样。我们报告说，吸附的纤维蛋白原水平接近 0.5 mg/m(2) 时会产生非特异性污垢。对于基本排斥性较小的下层表面，少量吸附的纤维蛋白原也会产生类似的效果。此外，观察到颗粒粘附急剧发生并且仅高于收集器上纤维蛋白原负载的阈值。另外，在离子强度范围内。 1，低于约0.05 M，由于静电排斥的屏蔽，1的增加减少了微粒捕获所需的纤维蛋白原。然而，令人惊讶的是，0.15 M 的离子强度完全降低了纤维蛋白原的吸附。这一观察结果与基于 DLVO 论点的预期相反，指出局部静电吸引力和水合效应驱动二氧化硅-纤维蛋白原粘附。这些行为以携带少量聚阳离子的二氧化硅表面上的微粒结合为基准，以深入了解静电在纤维蛋白原驱动的非特异性粘附中的作用。 (C) 2009 Elsevier B.V. 保留所有权利。
This work explores how long-range non-specific interactions, resulting from small amounts of adsorbed fibrinogen, potentially influence bioadhesion. Such non-specific interactions between protein adsorbed on a biomaterial and approaching cells or bacteria may complement or even dominate ligand-receptor mating. This work considers situations where the biomaterial surface and the approaching model cells (micron-scale silica particles) exhibit strong electrostatic repulsion, as may be the case in diagnostics and lab-on-chip applications. We report that adsorbed fibrinogen levels near 0.5 mg/m(2) produce non-specific fouling. For underlying surfaces that are less fundamentally repulsive, smaller amounts of adsorbed fibrinogen would have a similar effect. Additionally, it was observed that particle adhesion engages sharply and only above a threshold loading of fibrinogen on the collector. Also, in the range of ionic strength. 1, below about 0.05 M, increases in 1 reduce the fibrinogen needed for microparticle capture, due to screening of electrostatic repulsions. Surprisingly, however, ionic strengths of 0.15 M reduce fibrinogen adsorption altogether. This observation opposes expectations based on DLVO arguments, pointing to localized electrostatic attractions and hydration effects to drive silica-fibrinogen adhesion. These behaviors are benchmarked against microparticle binding on silica surfaces carrying small amounts of a polycation, to provide insight into the role of electrostatics in fibrinogen-driven non-specific adhesion. (C) 2009 Elsevier B.V. All rights reserved.