Development of a PCR-based mating-type assay for
Development of a PCR-based mating-type assay for
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开发基于 PCR 的交配型检测
DOI：
10.1016/j.femsle.2004.06.033
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发表时间：
2004
影响因子：
2.1
通讯作者：
A. Harã
中科院分区：
文献类型：
--
作者：
E. Yokoyama;K. Yamagishi;A. Harã
本研究开发了一种基于 PCR 的麦角皮科交配型检测方法。分别根据保守的α和HMG盒的氨基酸序列设计交配型基因MAT1-1-1和MAT1-2-1的PCR引物组。基于 PCR 的交配型测定可应用于各种麦角菌属（Balansia、Claviceps、Cordyceps 和 Epichloë）。大多数棒状真菌都具有 MAT1-1-1 或 MAT1-2-1，并且被认为是异宗合的。尽管交配型检测获得的PCR产物很短，但交配型基因的系统发育树比18S rDNA具有更好的分辨率，并且与其经济营养模式非常吻合。
This study developed a PCR-based mating-type assay forClavicipitaceae. PCR primer sets for the mating-type genesMAT1-1-1andMAT1-2-1were designed based on the amino acid sequences of the conserved alpha and HMG boxes, respectively. The PCR-based mating-type assay could be applied for various clavicipitaceous genera (Balansia, Claviceps, CordycepsandEpichloë). Most of the clavicipitaceous fungi possessed eitherMAT1-1-1orMAT1-2-1, and were supposed to be heterothallic. Although the PCR products obtained by the mating-type assay were short, the phylogenetic trees of the mating-type genes gave better resolutions than that of 18S rDNA and agreed well to their econutritional modes.