3D microfluidics via cyclic olefin polymer-based in situ direct laser writing
3D microfluidics via cyclic olefin polymer-based in situ direct laser writing
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DOI：
10.1039/c9lc00542k
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发表时间：
2019-09-07
期刊：
影响因子：
6.1
通讯作者：
Sochol, Ryan D.
中科院分区：
文献类型：
--
作者：
Alsharhan, Abdullah T.;Acevedo, Ruben;Sochol, Ryan D.
原位直接激光写入（isDLW）策略有助于将三维（3D）纳米结构组件直接打印到封闭微通道内部并完全密封到封闭微通道中，非常适合制造几何复杂的微流体技术。最近的努力已经证明了对isDLW使用微成型和结合方案的益处;然而，对聚二甲基硅氧烷（PDMS）的依赖导致有限的流体密封（例如，操作压力< 50-75 kPa）和与标准有机溶剂基显影剂的相容性差。为了绕过这些问题，在这里，我们探索使用环烯烃聚合物（COP）作为isDLW的微通道材料，通过研究与日益复杂程度相对应的三种基本类型的微流体系统：（i）“2.5D”功能性静态流体屏障（10-100 μ m高），在高达500千帕的输入压力下，支持不受影响的结构-通道密封;（ii）3D静态交织微血管启发结构（内径< 10 μ m），其表现出不同的荧光标记的微流体流动流的有效分离;以及（iii）3D动态致动微流体晶体管，其包括波纹状密封元件（壁厚= 500 nm），该密封元件可以通过施加的栅极压力主动变形以完全阻挡源极到漏极的流体流动。结合起来，这些结果表明，COP为基础的isDLW提供了一个有前途的途径，广泛的流体应用，需要显着的结构多功能性在亚微米尺度上与不变的密封完整性，如仿生器官芯片系统和集成微流体电路。
In situ direct laser writing (isDLW) strategies that facilitate the printing of three-dimensional (3D) nanostructured components directly inside of, and fully sealed to, enclosed microchannels are uniquely suited for manufacturing geometrically complex microfluidic technologies. Recent efforts have demonstrated the benefits of using micromolding and bonding protocols for isDLW; however, the reliance on polydimethylsiloxane (PDMS) leads to limited fluidic sealing (e.g., operational pressures < 50-75 kPa) and poor compatibility with standard organic solvent-based developers. To bypass these issues, here we explore the use of cyclic olefin polymer (COP) as an enabling microchannel material for isDLW by investigating three fundamental classes of microfluidic systems corresponding to increasing degrees of sophistication: (i) "2.5D" functionally static fluidic barriers (10-100 mu m in height), which supported uncompromised structure-tochannel sealing under applied input pressures of up to 500 kPa; (ii) 3D static interwoven microvesselinspired structures (inner diameters < 10 mu m) that exhibited effective isolation of distinct fluorescently labelled microfluidic flow streams; and (iii) 3D dynamically actuated microfluidic transistors, which comprised bellowed sealing elements (wall thickness = 500 nm) that could be actively deformed via an applied gate pressure to fully obstruct source-to-drain fluid flow. In combination, these results suggest that COP-based isDLW offers a promising pathway to wide-ranging fluidic applications that demand significant architectural versatility at submicron scales with invariable sealing integrity, such as for biomimetic organ-on-a-chip systems and integrated microfluidic circuits.