Geodermatophilus ruber sp. nov., isolated from rhizosphere soil of a medicinal plant.
Geodermatophilus ruber sp. nov., isolated from rhizosphere soil of a medicinal plant.
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DOI：
10.1099/ijs.0.020610-0
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发表时间：
2011
影响因子：
2.8
通讯作者：
Yu-Qin Zhang;Jie Chen;Hong-yu Liu;Yue-Qin Zhang;Wenjun Li;Li-Yan Yu
中科院分区：
文献类型：
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作者：
Yu-Qin Zhang;Jie Chen;Hong-yu Liu;Yue-Qin Zhang;Wenjun Li;Li-Yan Yu
从药用植物黄芪根际土壤中分离到一株放线菌，命名为CPCC 201356（T），并对其进行了多相分类学研究。在20-32 °C、pH 7.0-7.5和0- 1%（w/v）NaCl下发生良好生长。R2 A和ISP 2琼脂上的菌落为淡红色至红色，圆形，缺乏气生菌丝体;细胞粘附在琼脂上。肽聚糖含有内消旋-二氨基庚二酸作为诊断性二氨基酸。主要的甲基萘醌是MK-9（H（4））和MK-9。极性脂质由二磷脂酰甘油、磷脂酰乙醇胺和两种未知磷脂组成。主要的细胞脂肪酸为异C（16：0）、异C（15：0）和C（17：1）ω 8 c。基因组DNA的G+C含量为72.8mol%。基于16 S rRNA基因序列的系统发育分析表明，菌株CPCC 201356（T）属于Geodermatophilus科，与Geodermatophilus obscurus DSM 43160（T）形成一个独立的分支。该菌16 S rRNA基因序列与G. obscurus DSM 43160（T）.菌株CPCC 201356（T）与G.暗纹夜蛾DSM 43160（T）为17.4%。根据多相分类学研究的证据，一个新种，红色地皮嗜菌新种，模式菌株为CPCC 201356（T）（=DSM 45317（T）=CCM 7619（T））。
A novel actinobacterial strain, designated CPCC 201356(T), was isolated from a rhizosphere soil sample of the medicinal plant Astragalus membranaceus and subjected to a polyphasic taxonomic analysis. Good growth occurred at 20-32 °C, at pH 7.0-7.5 and with 0-1 % (w/v) NaCl. Colonies on R2A and ISP 2 agar were light red to red, round and lacked aerial mycelium; cells adhered to the agar. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H(4)) and MK-9. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. The major cellular fatty acids were iso-C(16 : 0), iso-C(15 : 0) and C(17 : 1)ω8c. The G+C content of the genomic DNA was 72.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CPCC 201356(T) belonged to the family Geodermatophilaceae and consistently formed a distinct sub-branch with Geodermatophilus obscurus DSM 43160(T). The organism showed 16S rRNA gene sequence similarity of 97.7 % with G. obscurus DSM 43160(T). DNA-DNA hybridization between strain CPCC 201356(T) and G. obscurus DSM 43160(T) was 17.4 %. On the basis of evidence from this polyphasic taxonomic study, a novel species, Geodermatophilus ruber sp. nov., is proposed; the type strain is CPCC 201356(T) (=DSM 45317(T) =CCM 7619(T)).