OBSERVATIONS ON HEARTS EXPLANTED IN VITRO FROM SNAIL AUSTRALORBIS GLABRATUS
OBSERVATIONS ON HEARTS EXPLANTED IN VITRO FROM SNAIL AUSTRALORBIS GLABRATUS
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DOI：
10.2307/3275797
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发表时间：
1963-01-01
影响因子：
1.3
通讯作者：
CHERNIN, E
中科院分区：
文献类型：
--
作者：
CHERNIN, E
描述了血淋巴分析，技术程序和培养方案，使整个研究成为可能，胰蛋白酶处理过的心脏在塑料培养瓶中外植。两种类型的细胞，变形细胞和上皮细胞，从外植体中出现，并在不同的时间内保持明显的良好状态。未观察到细胞分裂。移植的心脏发生了一定的外观和组织学改变，但器官在体外保持了基本的完整性，并且在移植后至少47天观察到心跳。
Hemolymph analyses, technical procedures, and culture solutions are described which made possible the study of whole, trypsin-treated hearts of Australorbis glabratus explanted in plastic culture flasks. Cells of two types, amebocytic and "epithelial," emerged from explants and persisted in apparently good condition for varying lengths of time. Cell division was not observed. The explanted hearts underwent certain gross and histologic alterations, but the organs retained their essential integrity in vitro, and heartbeat was observed for at least 47 days after explantation.