Dual protecting encapsulation synthesis of ultrastable quantum-dot nanobeads for sensitive and accurate detection of cardiac biomarkers
Dual protecting encapsulation synthesis of ultrastable quantum-dot nanobeads for sensitive and accurate detection of cardiac biomarkers
复制标题
双重保护封装合成超稳定量子点纳米珠，用于灵敏准确地检测心脏生物标志物
DOI：
10.1016/j.snb.2021.130275
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发表时间：
2021-06-15
影响因子：
8.4
通讯作者：
Li, Lin Song
中科院分区：
文献类型：
--
作者：
Li, Jinjie;Lv, Yanbing;Li, Lin Song
标记材料是决定横向流动免疫分析法（LFIA）准确灵敏检测能力的关键因素。本文首次开发了一种聚乙烯吡咯烷酮（PVP）缠绕和SiO2涂层的双重保护策略，用于生成具有高荧光和非凡稳定性的量子点beads@SiO2-COOH （QBs@SiO2-COOH）纳米珠，大大提高了快速LFIA的灵敏度和稳定性。QBs@SiO2-COOH在复杂基质中表现出优异的胶体稳定性，发光强度是对应量子点的1967倍。以合成的QBs@SiO2-COOH为报告蛋白构建新型LFIA纳米传感器，仅需60 μ L样品即可在10 min内实现心肌肌钙蛋白I （cTnI）的快速定量检测。检出限为0.036 ng mL-1，在0.12 ~ 125 ng mL-1之间呈良好的线性关系。重要的是，该检测方法具有良好的重复性（CV < 8%）和优越的稳定性，首次通过检测不同温度保存120天的临床血清样品证明了这一点。此外，所开发的纳米传感器实现了cTnI、肌酸激酶- mb （CC-MB）和肌红蛋白（Myo）的同时定量检测。该QBs@SiO2-COOH-based快速LFIA纳米传感器在体外诊断生物标志物的快速、稳定、灵敏检测方面具有良好的前景。
The labeling material is a key factor that determines the ability of lateral-flow immunoassay (LFIA) for accurate and sensitive detection. Herein, a dual protection strategy with both polyvinylpyrrolidone (PVP) winding and SiO2 coating has been first developed for generating quantum dot beads@SiO2-COOH (QBs@SiO2-COOH) nanobeads with high fluorescence and extraordinary stability, which greatly improved the sensitivity and stability of rapid LFIA. QBs@SiO2-COOH showed outstanding colloidal stability in complex matrices and 1,967-fold higher luminescence than that of corresponding QDs. The novel LFIA nanosensor was constructed by utilizing synthesized QBs@SiO2-COOH as the reporter to realize rapid quantitative detection of cardiac troponin I (cTnI) within 10 min using only 60 mu L of sample. The limit of detection (LOD) was 0.036 ng mL-1 with the excellent linearity relationship between 0.12 and 125 ng mL-1. Importantly, the detection method owned good reproducibility (CV < 8 %) and predominant stability, which were demonstrated for the first time by detecting clinical serum samples preserved at different temperature in 120 days. Moreover, the developed nanosensor achieved the simultaneous quantitative detection of cTnI, creatine kinase-MB (CC-MB), and myoglobin (Myo). This QBs@SiO2-COOH-based rapid LFIA nanosensor holds a good prospect for rapid, stable and sensitive detection of biomarker for in vitro diagnostics.