Characterization of radiation-induced damage in d(TpApCpG).
Characterization of radiation-induced damage in d(TpApCpG).
复制标题
d(TpApCpG) 中辐射引起的损伤的表征。
DOI：
10.1080/09553009014552141
复制
发表时间：
1990
影响因子：
2.6
通讯作者：
Box,HC
中科院分区：
文献类型：
--
作者：
Paul,CR;Budzinski,EE;Maccubbin,A;Wallace,JC;Box,HC
The radiation chemistry of the oligomer d(TpApCpG) X-irradiated in aqueous solution containing glutathione was studied. Four products were isolated by HPLC and characterized by NMR spectroscopy. Two of the major products are isomers of a 5-hydroxy-5,6-dihydrothymine modification of d(TpApCpG). A dihydrothymine modification is also formed. The other major product is a result of strand scission. These products are different from the major products identified previously in a study of d(TpApCpG) X-irradiated in oxygenated solution. The effect of specific radiation-induced lesions on the behaviour of d(TpApCpG) as substrate to a spleen phosphodiesterase-micrococcal nuclease combination of enzymes and to nuclease P1 was studied. These enzymes are of interest because they are used in postlabelling assays of DNA damage.
