Tonotopic action potential tuning of maturing auditory neurons through endogenous ATP
Tonotopic action potential tuning of maturing auditory neurons through endogenous ATP
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DOI：
10.1113/jp273272
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发表时间：
2017-02-01
影响因子：
5.5
通讯作者：
Milenkovic, Ivan
中科院分区：
文献类型：
--
作者：
Jovanovic, Sasa;Radulovic, Tamara;Milenkovic, Ivan
突触的完善和加强是活动依赖的过程，在整个中央听觉系统中建立有序排列的耳蜗图。听觉脑干回路的成熟是由发育中的耳蜗内毛细胞产生的动作电位（ap）引导的。发育中的中央听觉神经元的AP放电可受旁分泌ATP信号的调节，如耳蜗核丛状细胞和梯形体内侧核的主神经元。然而，目前尚不清楚神经元活动是否会受到核张力位置（即声音频率选择性）的特异性调节。使用听力发作前的切片记录和听力发作后使用离子吸入性药物的体内记录，我们发现发育中的沙鼠耳蜗腹核中细胞特异性嘌呤能调节遵循精确的音调变化模式。在高频区域，ATP反应性在听力发作前减弱。在低频至中频区域，ATP调节在听力发作后持续存在于低频丛状细胞（特征频率）中
Synaptic refinement and strengthening are activity-dependent processes that establish orderly arranged cochleotopic maps throughout the central auditory system. The maturation of auditory brainstem circuits is guided by action potentials (APs) arising from the inner hair cells in the developing cochlea. The AP firing of developing central auditory neurons can be modulated by paracrine ATP signalling, as shown for the cochlear nucleus bushy cells and principal neurons in the medial nucleus of the trapezoid body. However, it is not clear whether neuronal activity may be specifically regulated with respect to the nuclear tonotopic position (i.e. sound frequency selectivity). Using slice recordings before hearing onset and in vivo recordings with iontophoretic drug applications after hearing onset, we show that cell-specific purinergic modulation follows a precise tonotopic pattern in the ventral cochlear nucleus of developing gerbils. In high-frequency regions, ATP responsiveness diminished before hearing onset. In low-to-mid frequency regions, ATP modulation persisted after hearing onset in a subset of low-frequency bushy cells (characteristic frequency