The role of NF-1 factors in regulation of elastin gene transcription
The role of NF-1 factors in regulation of elastin gene transcription
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DOI：
10.1016/s0945-053x(99)00020-7
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发表时间：
1999-06-01
期刊：
影响因子：
6.9
通讯作者：
Foster, JA
中科院分区：
文献类型：
--
作者：
Degterev, A;Foster, JA
在新生大鼠主动脉平滑肌细胞和肺成纤维细胞培养中，人类弹性蛋白启动子的- 195至- 500 bp区域显示出高活性。此外，该区域还涉及控制这两种细胞类型中弹性蛋白转录的差异基础水平。本研究的总体目标是确定-195- -500-bp区域内的阳性因子，并确定与该序列结合的反式作用因子。缺失和连接子扫描的组合突变分析将阳性元件定位在-401和-415 bp之间。凝胶位移分析表明，阳性元素结合NF-1家族成员。在果蝇Schneider细胞中共转染CTF1表达载体显示NF-1家族成员通过该位点激活弹性蛋白启动子活性的能力。对SMC和肺成纤维细胞分离的核提取物进行Western和Southwestern blot比较分析，为不同NF-1家族成员的细胞特异性表达可能对弹性蛋白转录水平的差异调节奠定了基础。(C) 1999 Elsevier Science B.V./International Society of Matrix Biology。版权所有。
The - 195- to - 500-bp region of the human elastin promoter has been shown to convey high activity in neonatal rat aortic smooth muscle cell and pulmonary fibroblast cell cultures. In addition, this region has been implicated in controlling the differential basal level of elastin transcription in these two cell types. The overall goal of this study was to define the positive element(s) within the -195- to -500-bp region and to identify the trans-acting factors binding to this sequence. A combination of deletion and linker scan mutational analyses localizes the positive element between -401 and -415 bp. Gel shift analyses demonstrate that the positive element binds NF-1 family members. Co-transfection of a CTF1 expression vector in Drosophila Schneider cells shows the ability of an NF-1 family member to activate elastin promoter activity through this site. Comparative Western and Southwestern blot analyses of nuclear extracts isolated from SMC and lung fibroblasts lay the foundation for possible differential regulation of elastin transcriptional levels via cell specific expression of different NF-1 family members. (C) 1999 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.