IP-10, MCP-1, MCP-2, MCP-3, and IL-1RA hold promise as biomarkers for infection with M. tuberculosis in a whole blood based T-cell assay.
IP-10, MCP-1, MCP-2, MCP-3, and IL-1RA hold promise as biomarkers for infection with M. tuberculosis in a whole blood based T-cell assay.
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IP-10，MCP-1，MCP-2，MCP-3和IL-1RA在整个血液基的T细胞测定法中作为结核分枝杆菌感染的生物标志物具有希望。
DOI：
10.1186/1756-0500-2-19
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发表时间：
2009-02-04
影响因子：
1.8
通讯作者：
Ravn P
中科院分区：
文献类型：
--
作者：
Ruhwald M;Bjerregaard-Andersen M;Rabna P;Eugen-Olsen J;Ravn P
对结核分枝杆菌抗原的 IFN-γ 反应被用作结核感染的体外诊断测试。这些测试具有高度特异性，但敏感性可能因免疫抑制而受损。这项小型探索性研究的目的是将用于结核病体外诊断的三种新型生物标志物（MCP-1、MCP-3 和 IL-1RA）与当前已建立的生物标志物 IFN-γ 以及新描述的 IP-10 和 MCP-2 进行比较。来自 8 名活动性结核病患者和 7 名健康对照者的全血在 Quantiferon In Tube 试管中用结核分枝杆菌特异性抗原和丝裂原进行刺激。使用 Luminex 和 ELISA (IFN-γ) 测量生物标志物的水平。我们发现与 IFN-γ 相比，所有五种新生物标志物的表达浓度显着更高。与对照组相比，患者未刺激样本中的 IP-10 和 MCP-3 水平较高。所有生物标志物都具有诊断潜力，因为它们可以区分患者和对照组。 IP-10 和 MCP-2 似乎最有前途，因为它们在抗原刺激下高水平表达，而在未刺激的样品中表达水平较低。需要进一步的研究来探索这些高表达的新型生物标志物单独和组合的潜力。
IFN-γ responses to M. tuberculosis antigens are used as in-vitro diagnostic tests for tuberculosis infection. The tests are highly specific but sensitivity may be impaired due to immuno-suppression. The objective of this small exploratory study was to compare three novel biomarkers for in-vitro diagnosis of tuberculosis – MCP-1, MCP-3 and IL-1RA – with the current established biomarker IFN-γ and the newly described IP-10 and MCP-2. Whole blood from 8 patents with active tuberculosis and from 7 healthy controls was stimulated with M. tuberculosis specific antigens and mitogen in the Quantiferon In Tube test tubes. Levels of biomarkers were measured using Luminex and ELISA (IFN-γ). We found all five new biomarkers were expressed in significantly higher concentrations compared to IFN-γ. IP-10 and MCP-3 levels in the un-stimulated samples were higher in patients compared with controls. All biomarkers had diagnostic potential as they could differentiate between the patients and the controls. IP-10 and MCP-2 seemed most promising as they were expressed in high levels with antigen stimulation and were low in the un-stimulated samples. Further studies are needed to explore the potential of these highly expressed novel biomarkers individually and in combination.