PCR amplification and sequence analyses of ITS-1 rDNA from Cryptosporidium andersoni in dairy cattle
PCR amplification and sequence analyses of ITS-1 rDNA from Cryptosporidium andersoni in dairy cattle
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DOI：
10.1007/s00436-006-0358-x
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发表时间：
2007-04
影响因子：
2
通讯作者：
R. Zhou;Guoqing Li;S. Xiao;Yanxun Xia;Y. Guo
中科院分区：
文献类型：
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作者：
R. Zhou;Guoqing Li;S. Xiao;Yanxun Xia;Y. Guo
为了探讨安氏隐孢子虫（Cryptosporidiumandersoni）GD、HN和AH株核糖体DNA（rDNA）内转录间隔区1（ITS-1）是否可作为遗传标记，对GD、HN和AH株的ITS-1进行了扩增和测序。andersoni在中国的不同地理起源，并区分C. andersoni与其他隐孢子虫属种的区别。结果表明，GD、HN、AH株的ITS-1序列基本一致，与GenBank中已登录的鼠隐孢子虫和微小隐孢子虫的ITS-1序列存在明显差异，而AH株的ITS-1序列与其它两株存在3个碱基的差异。我们的研究表明ITS-1序列为C. andersonispecies，为隐孢子虫病的诊断奠定了基础。
The internal transcribed spacer 1 (ITS-1) of the ribosomal DNA (rDNA) from GD, HN, and AH strains ofCryptosporidium andersoniwas amplified and sequenced to assess whether the ITS-1 rDNA could be used as genetic markers ofC. andersonifrom different geographic origins in China and to differentiateC. andersonifrom otherCryptosporidiumspecies. The result showed that the ITS-1 sequences of GD, HN, and AH strains were basically identical, which were unequivocally different with the sequences of theCryptosporidium murisandCryptosporidium parvumregistered in the GenBank; however, the ITS-1 sequence of the AH strain differed at three bases compared with that of the other two strains. Our study indicates that the ITS-1 sequences provide useful genetic markers for the identification and differentiation ofC. andersonispecies and also lay down the foundation for diagnostics of cryptosporidiosis.