Detection of catechol using an electrochemical biosensor based on engineered Escherichia coli cells that surface-display laccase
Detection of catechol using an electrochemical biosensor based on engineered Escherichia coli cells that surface-display laccase
复制标题
使用基于表面展示漆酶的工程大肠杆菌细胞的电化学生物传感器检测儿茶酚
DOI：
10.1016/j.aca.2018.01.008
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发表时间：
2018
影响因子：
6.2
通讯作者：
Li Lin
中科院分区：
文献类型：
--
作者：
Zhang Zhen;Liu Jin;Fan Jin;Wang Zhiyong;Li Lin
In this study, we report an electrochemical microbial biosensor that was made by immobilizing a bacterial laccase on the surface ofEscherichia colicells followed by adsorption of modified live cells onto a glassy-carbon electrode. Expression and surface localization of laccase on target cells were confirmed by Western blotting, flow cytometry assays and immunofluorescence microscopy observation. Increased tandem-aligned anchors with three repeats of the N-terminal domain of an ice nucleation protein were used to construct a highly activeE. coliwhole cell laccase-based catalytic system. When the proposed biosensor was used to detect catechol, the electrochemical response under optimized pH conditions was linear within a concentration range of 0.5 μM–300.0 μM catechol. Metal ions (Mn2+, Fe3+, Cu2+, Mg2+, Al3+and Zn2+) at concentrations from 1 to 10 mg L−1, bovine serum albumin and glucose at concentrations from 0.1 to 10 g L−1, and ascorbic acid at concentrations from 0.01 to 0.1 g L−1did not cause a noticeable interference effect. The detection limit of 0.1 μM catechol was comparable to those of other biosensors based on purified chemically modified laccases. When used to detect catechol in real red wine and tea samples, the biosensor offered a considerable level of accuracy comparable to the HPLC method as well as high recovery rates (98.2%–103.8%) towards all of the tested samples. Moreover, the developed system also exhibited high stability and reproducibility.
