Metal associations to microparticles, nanocolloids and macromolecules in compost leachates: Size characterization by asymmetrical flow field-flow fractionation coupled to ICP-MS
Metal associations to microparticles, nanocolloids and macromolecules in compost leachates: Size characterization by asymmetrical flow field-flow fractionation coupled to ICP-MS
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DOI：
10.1016/j.aca.2009.12.021
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发表时间：
2010-02-28
影响因子：
6.2
通讯作者：
Castillo, J. R.
中科院分区：
文献类型：
--
作者：
Bolea, E.;Laborda, F.;Castillo, J. R.
提出了一种基于非对称流动-流动分馏(AsFIFFF)表征环境微粒(粒径大于1µm)、纳米胶体(1~15 nm)和大分子(小于1000 kDa)尺寸的方法，该方法利用了常规和空间洗脱模式。对程序进行了优化，以最大限度地减少表征物种的大小分布和金属缔合的潜在变化。在用ASFIFFF分离之前，样品要经过固体悬浮液的重力沉降，然后对沉淀后的样品进行离心。通过比较沉淀物和离心物的断口图谱，可以对在AsFIFFF系统中以立体模式洗脱的微粒进行表征。通过在AsFIFFF系统中正常模式下施加不同的操作条件，对离心样品进行了纳米胶体和大分子的表征。通过0.45微米孔径的膜与传统的前向过滤的比较表明，过滤可以去除低于其标称孔径的颗粒，改变了样品相对于离心法的粒度分布。该方法已被应用于堆肥渗滤液的粒度表征。通过将AsFIFFF系统与电感耦合等离子体质谱仪(ICPMS)联用，确定了这三种不同组分对金属活化的贡献。(C)2009爱思唯尔B.V.保留所有权利。
A methodological approach based on the size characterization of environmental microparticles (size larger than 1 mu m), nanocolloids (1 mu m to 15 nm) and macromolecules (lower than 1000 kDa) by asymmetrical flow field-flow fractionation (AsFIFFF), taking advantage of both normal and steric elution modes, is presented. The procedure was optimized to minimize the potential alteration of the size distribution and metal associations of the species characterized. Prior to separation by AsFIFFF, samples are subjected to gravitational settling of the solid Suspension, followed by a centrifugation of the settled sample. The comparison between the fractograms of the settled and the centrifuged samples allows the characterization of the microparticles, which are eluted in steric mode in the AsFIFFF system. The characterization of nanocolloids and macromolecules is carried out on the centrifuged sample by applying different operational conditions under normal mode in the AsFIFFF system. A comparison with the conventional frontal filtration through 0.45 mu m pore size membranes have shown that filtration removes particles below their nominal pore size, modifying the size distribution of the samples respect to the centrifugation. The methodology proposed has been applied to the size characterization of compost leachates. The contribution of these three differentiated fractions to the mobilization of metals has been determined by coupling the AsFIFFF system to an inductively Coupled plasma mass spectrometer (ICP-MS). (C) 2009 Elsevier B.V. All rights reserved.