Shelter covering altered polyamines accumulation of cherry (Prunus pseudocerasus Lindl.) via elevating the expression of spermidine synthase (SPDS) gene
Shelter covering altered polyamines accumulation of cherry (Prunus pseudocerasus Lindl.) via elevating the expression of spermidine synthase (SPDS) gene
复制标题
遮蔽物通过提高亚精胺合成酶（SPDS）基因的表达改变了樱桃（Prunus pseudocerasus Lindl.）多胺积累
DOI：
10.1016/j.scienta.2020.109440
复制
发表时间：
2020-08
影响因子：
4.3
通讯作者：
Min-Feng Wu;T. Tian;Houyu Liu;Huimin Zhang;Z. Qiu;G. Qiao;X. Wen
中科院分区：
文献类型：
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作者：
Min-Feng Wu;T. Tian;Houyu Liu;Huimin Zhang;Z. Qiu;G. Qiao;X. Wen
Polyamines (PAs), including putrescine (Put), spermidine (Spd), and spermine (Spm), play key roles in plant growth, development, and abiotic/biotic stress tolerance. Their functions appear to be intimately related to their synthesis, which occurs via arginine/ornithine decarboxylase (ADC/ODC), Spd synthase (SPDS) and Spm synthase (SPMS) etc. Previously, the transcriptional regulation of cherry under the shelter covering was carried out, and the PA synthase-related genes, particularlyspermidine synthase(SPDS) gene, were differentially expressed in the fruits as exposure to the shelter covering. In the present work, the full-length cDNA sequence of this gene was cloned and named asCpSPDS(GenBank accession: MK045261). Bioinformatics analysis indicated that the cDNA sequence ofCpSPDSwas 1243 bp in length. Cluster analysis showed thatCpSPDShad the highest homologies with those of sweet cherry (XP 021826297, 100 %) and peach (XP 020413638, 99 %). Subcellular localization detected that target gene was located in both nucleus and cytoplasm. The qPCR quantification showed thatCpSPDSwas differentially expressed in alabastrums, fruitlets, mature fruits and mature leaves of cherry. Interestingly, shelter covering significantly improved the fruitsetting in comparison with those from the shelter-free, gave a positive effect on the expression ofCpSPDSgene. The endogenous free PAs were quantified in different tissues by high-performance liquid chromatography (HPLC). The PAs contents of the sheltered trees,i.e.Put, Spm and Spd were diversely higher in comparison to the shelter-free, especially in alabastrums, whose Spd titer of the sheltered was 3.29 folds higher than that of the unsheltered, which somehow collaborated result of mRNA quantification. Therefore, shelter covering for cherry might substantially enhance the expression ofCpSPDSgene, leading to altering PAs accumulation, which at least partially contributed to the elevation in fruitsetting in rainy regions.
