Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response
Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response
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DOI：
10.3389/fpls.2019.00067
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发表时间：
2019-02
影响因子：
5.6
通讯作者：
Peipei Xu;Weiming Cai
中科院分区：
文献类型：
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作者：
Peipei Xu;Weiming Cai
脱落酸（阿坝）除了调节种子休眠和叶片衰老外，还参与植物对各种环境胁迫的适应。ABI 3是一个含有B3结构域的家族蛋白，在种子发育过程中的阿坝信号通路中发挥作用。到目前为止，还没有在甘蓝型油菜中研究ABI 3直链淀粉。本研究旨在探讨BnABI 3在植物发育和逆境响应中的作用。在这里，我们确定了一个拟南芥线（GS 1）从人口的诱变种子，并表明GS 1是一个新的等位基因AtABI 3。当用BnABI 3基因转化拟南芥gs1突变体时，转化的植物产生变黄的种子并获得耐干燥性。此外，BnABI 3通过直接靶向AtMUM 1和AtGATL 5基因来调节种皮发育和粘液分泌。此外，我们发现，BnABI 3的表达拯救了gs1冷冻诱导的绿色种子着色的转基因拟南芥AtSGR 1/2为目标。BnABI 3还参与侧根的发育，并赋予阿坝和生长素信号在根中的新的相互作用。还测试了ABI 3蛋白在内质网稳态中的潜在作用。总之，我们的研究结果表明，BnABI 3介导植物发育和胁迫反应。
Abscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses in addition to the regulation of seed dormancy and leaf senescence. ABI3 is a B3 domain-containing family protein and functions in the ABA signaling pathway during seed development. To date, the ABI3 orthologous have not been studied in Brassica napus. The aim of this study is to investigate the function of BnABI3 in plant development and stress response. Here, we identified an Arabidopsis line (gs1) from a population of mutagenized seeds and showed that GS1 is a new allele of AtABI3. When the Arabidopsis gs1 mutant was transformed with the BnABI3 gene, the transformed plants produced seeds that turned yellow and acquired desiccation tolerance. Moreover, BnABI3 regulates seed coat development and mucilage secretion by directly targeting the AtMUM1 and AtGATL5 genes. In addition, we showed that BnABI3 expression rescued gs1 freezing-induced green seed coloration by targeting AtSGR1/2 in transgenic Arabidopsis. BnABI3 is also involved in lateral root development and conferred a novel interaction between ABA and auxin signaling in roots. The potential role of ABI3 protein in endoplasmic reticulum homoeostasis was also tested. Altogether, our results indicated that BnABI3 mediates both plant development and the stress response.