The effect of hydrostatic pressure on model membrane domain composition and lateral compressibility
The effect of hydrostatic pressure on model membrane domain composition and lateral compressibility
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DOI：
10.1039/c5cp04239a
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发表时间：
2016-01-07
影响因子：
3.3
通讯作者：
Brooks, N. J.
中科院分区：
文献类型：
--
作者：
Barriga, H. M. G.;Law, R. V.;Brooks, N. J.
三元模型膜中的相分离是在一定的温度和组成范围内发生的，并且可以通过增加静水压力来诱导。我们用小角X射线散射(SAXS)研究了二油酰磷脂酰胆碱(DOPC)、二棕榈酰磷脂酰胆碱(DPPC)和胆固醇(CHOL)混合物中沿预定连接线的相分离。我们可以毫不含糊地区分两相混合物衍射图中的液体有序相(L-o)和液体无序相(L-d)，并比较它们的横向可压缩性。在大气压和高达100 Mpa的压力下，测定了连接线端点随静水压力的变化。我们发现，随着压力的升高，结线向相图的DOPC富集区延伸和移动，这一行为与报道的温度降低时的行为略有不同。
Phase separation in ternary model membranes is known to occur over a range of temperatures and compositions and can be induced by increasing hydrostatic pressure. We have used small angle X-ray scattering (SAXS) to study phase separation along pre-determined tie lines in dioleoylphosphatidylcholine (DOPC), dipalmitoylphosphatidylcholine (DPPC) and cholesterol (CHOL) mixtures. We can unequivocally distinguish the liquid ordered (L-o) and liquid disordered (L-d) phases in diffraction patterns from biphasic mixtures and compare their lateral compressibility. The variation of tie line endpoints with increasing hydrostatic pressure was determined, at atmospheric pressure and up to 100 MPa. We find an extension and shift of the tie tines towards the DOPC rich region of the phase diagram at increased pressure, this behaviour differs slightly from that reported for decreasing temperature.