Epi-fluorescence microscopy and 3 D printing : An easily implemented approach for producing accurate physical models of micro-and macroscopic biological samples
Epi-fluorescence microscopy and 3 D printing : An easily implemented approach for producing accurate physical models of micro-and macroscopic biological samples
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落射荧光显微镜和 3D 打印：一种易于实施的方法，用于生成微观和宏观生物样品的精确物理模型
DOI：
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发表时间：
2017
期刊：
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通讯作者：
M. Savoian
中科院分区：
文献类型：
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作者：
K. Holt;M. Savoian
生命科学专业的学生遇到的一个常见困难是无法可视化小（微米到毫米级）复杂生物标本的三维（3D）性质。大多数表示都是二维（2D）的，不能明确地传达复杂的3D分布或纹理。在这里，我们概述了通过扫描共聚焦显微镜（SCM）和3D热塑性打印相结合生成触觉模型的方法。该方法的多功能性证明使用三个高度不同的样品不同的规模和几何形状：前期细胞核（亚细胞水平），花粉壳（细胞水平），最后，果蝇（小生物体水平）。图像采集，数据处理和物理打印的考虑进行了讨论。
A common difficulty experienced by students of the life sciences is the inability to visualise the three dimensional (3D) nature of small (micrometer to millimetre-scale) complex biological specimens. Most representations are two dimensional (2D) and cannot unambiguously convey complex 3D distributions or textures. Here we outline an approach for generating tactile models through the combination of scanning confocal microscopy (SCM) and 3D thermoplastic printing. The versatility of the approach is demonstrated using three highly divergent samples of different scales and geometries: prophase nuclei (subcellular level), pollen shells (cellular level) and finally, fruit flies (small organism level). Considerations for image acquisition, data handling and physical printing are discussed.