Expression of p63 and p73 in ameloblastomas
Expression of p63 and p73 in ameloblastomas
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DOI：
10.1111/j.1600-0714.2005.00284.x
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发表时间：
2005-04-01
影响因子：
3.3
通讯作者：
Ooya, K
中科院分区：
文献类型：
--
作者：
Kumamoto, H;Ohki, K;Ooya, K
背景：为探讨P53同系物在牙源性肿瘤发生和分化中的作用，对P63和P73在成釉细胞瘤和牙胚组织中的表达进行了分析。方法：采用免疫组织化学和逆转录聚合酶链式反应(RT-PCR)方法检测9例牙胚性成釉细胞瘤、48例良性成釉细胞瘤和5例恶性成釉细胞瘤组织中P63和P73的表达。促结缔组织增生性成釉细胞瘤P63表达显著高于棘瘤和颗粒细胞成釉细胞瘤，成釉细胞癌P63表达高于转移性成釉细胞瘤。P73在丛状成釉细胞瘤中的表达明显高于滤泡性成釉细胞瘤，基底细胞成釉细胞瘤的表达明显高于颗粒细胞成釉细胞瘤。DeltaNp63和TAp73在所有牙源性发育和肿瘤组织中均有表达。TAp63mRNA在8个牙胚、16个成釉细胞瘤和1个恶性成釉细胞瘤中表达，而DeltaNp73在8个牙胚、9个成釉细胞瘤和1个恶性成釉细胞瘤中表达。主要表达的亚型的变化表明，p63和p73在牙源性组织中可能具有不同的功能。
BACKGROUND: To clarify the role of p53 homologs in oncogenesis and cytodifferentiation of odontogenic tumors, expression of p63 and p73 was analyzed in ameloblastomas as well as tooth germs.METHODS: Tissue specimens of nine tooth germs and 48 benign and five malignant ameloblastomas were examined by immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) for the expression of p63 and p73.RESULTS: Immunoreactivity for p63 and p73 was evident in epithelial cells neighboring the basement membrane in developing and neoplastic odontogenic tissues. p63 expression in desmoplastic ameloblastomas was significantly higher than in acanthomatous and granular cell ameloblastomas, and ameloblastic carcinomas showed higher p63 expression than metastasizing ameloblastomas. p73 expression was significantly higher in plexiform ameloblastomas than in follicular ameloblastomas, and basal cell ameloblastomas showed higher p73 expression than granular cell ameloblastomas. mRNA transcripts for DeltaNp63 and TAp73 were detected in all developing and neoplastic odontogenic tissues. TAp63 mRNA was expressed in five of eight tooth germs, 16 of 34 ameloblastomas, and one of one malignant ameloblastoma, whereas DeltaNp73 mRNA was recognized in one of eight tooth germs, nine of 34 ameloblastomas, and one of one malignant ameloblastoma.CONCLUSION: The expression of p63 and p73 suggests that these p53 homologs play a role in differentiation and proliferation of odontogenic epithelial cells. Variations of predominantly expressed isoforms suggest that p63 and p73 might differentially function in odontogenic tissues.