ISOLATION AND CHARACTERIZATION OF THE M-EAEI MODIFICATION METHYLASE
ISOLATION AND CHARACTERIZATION OF THE M-EAEI MODIFICATION METHYLASE
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DOI：
10.1042/bj2380613
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发表时间：
1986-09-01
影响因子：
4.1
通讯作者：
BROWN, NL
中科院分区：
文献类型：
--
作者：
JACOBS, D;BROWN, NL
从产气肠杆菌PW 201中部分纯化了与限制性内切酶Eae I对应的修饰酶（M. Eae I）。M.EaeI酶将构成EaeI识别位点的相关序列家族的每条链中最内侧的胞嘧啶残基甲基化，得到：5“-Y-G-G-5 mC-C-R-3”，其中5 mC是5-甲基胞嘧啶。M.EaeI保护这些位点不被HaeIII切割，并保护重叠的5“-C-C-G-G-3”位点不被HpaII和MspI切割。
The modification enzyme (M.EaeI) corresponding to the restriction endonuclease EaeI was partially purified from Enterobacter aerogenes PW201. The M.EaeI enzyme methylates the innermost cytosine residue in each strand of the family of related sequences that constitute the EaeI recognition site to give: 5''-Y-G-G-5mC-C-R-3'' where 5mC is 5-methycytosine. M.EaeI protects these sites against cleavage by HaeIII, and protects overlapping 5''-C-C-G-G-3'' sites against cleavage by both HpaII and MspI.