Application of the Johnson-Kendall-Roberts model in AFM-based mechanical measurements on cells and gel.
Application of the Johnson-Kendall-Roberts model in AFM-based mechanical measurements on cells and gel.
复制标题
DOI：
10.1016/j.colsurfb.2015.06.044
复制
发表时间：
2015-10-01
影响因子：
5.8
通讯作者：
Shaitan, K. V.
中科院分区：
文献类型：
--
作者：
Efremov, Yu. M.;Bagrov, D. V.;Shaitan, K. V.
由原子力显微镜（AFM）与胶体探针获得的力-距离曲线（FC）包含有关样品的粘弹性和粘附性的信息。在这里，我们处理了在聚丙烯酰胺凝胶（在水或PBS中）和Vero细胞（在培养基中）上获得的FC的方法和回缩部分。将约翰逊Kendall Roberts模型应用于回缩曲线以解释粘附。加载速率，保持时间和压痕深度的粘附力和杨氏模量，计算的方法和回缩曲线的影响进行了研究。结果表明，体积和局部界面粘弹性可以影响所观察到的方法回缩滞后和测量参数。加入1%的牛血清白蛋白（BSA）降低了探针与PAA凝胶表面的粘附力，从而减弱了界面粘弹性效应。相反，Vero细胞的荧光强度在加入BSA后增加，表明细胞-探针相互作用的复杂性。(C)2015 Elsevier B. V.版权所有。
The force-distance curves (FCs) obtained by the atomic force microscope (AFM) with colloid probes contain information about both the viscoelastic properties and adhesion of a sample. Here, we processed both the approach and retraction parts of FCs obtained on polyacrylamide gels (in water or PBS) and Vero cells (in a culture medium). The Johnson Kendall Roberts model was applied to the retraction curves to account for the adhesion. The effects of loading rate, holding time and indentation depth on adhesion force and Young's modulus, calculated from approach and retraction curves, were studied. It was shown that both bulk and local interfacial viscoelasticity can affect the observed approach-retraction hysteresis and measured parameters. The addition of 1% bovine serum albumin (BSA) decreased adhesion of the probe to the PAA gel surface, so interfacial viscoelasticity effects were diminished. On the contrary, the adhesiveness of Vero cells increased after BSA addition, indicating the complex nature of the cell-probe interaction. (C) 2015 Elsevier B.V. All rights reserved.