Streptomyces triticagri sp. nov. and Streptomyces triticirhizae sp. nov., two novel Actinobacteria isolated from the rhizosphere soil of wheat (Triticum aestivum L.)
Streptomyces triticagri sp. nov. and Streptomyces triticirhizae sp. nov., two novel Actinobacteria isolated from the rhizosphere soil of wheat (Triticum aestivum L.)
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小小麦链霉菌 sp.
DOI：
10.1099/ijsem.0.003727
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发表时间：
2020
影响因子：
2.8
通讯作者：
Wang Xiangjing
中科院分区：
文献类型：
--
作者：
Han Chuanyu;Yu Zhiyin;Zhao Junwei;Shi Haoran;Hu Jiangmeihui;Yu Bing;Song Jia;Shen Yue;Xiang Wensheng;Wang Xiangjing
从河南驻马店小麦根际土壤中分离到两株新型放线菌，分别命名为neau - yy421t和NEAU-YY642T，并采用多相法对其进行了鉴定。基于16S rRNA基因序列的系统发育分析表明，菌株neau - yy421t和neau - yy642t同属链霉菌属，与富链霉菌cgmcc 4.1732T（序列相似性为97.9%）、长链霉菌idsm 40282T（序列相似性为97.8%）、与赵州链霉菌islzs - 5t（序列相似性为98.0%）和sediym 65188T（序列相似性为97.5%）亲缘关系最为密切。两株细胞壁均含有二氨基戊酸作为诊断二氨基酸，全细胞水解产物为葡萄糖和核糖。对streptomyces属的theatpD、gyrB、recA、rpoBandtrpBgenes进行多位点序列分析，结果表明，这两个菌株在streptomyces属中形成了独立的分支。此外，结合DNA-DNA杂交结果和培养和生理特性表明，这两个菌株可以与它们最接近的系统发育亲缘菌株区分开来。因此，菌株neau - yy421和neau - yy642属于链霉菌属的两个新种，并将其命名为streptomyces triticagrisp。[11 .] (NEAU-YY421T=CGMCC 4.7476T=DSM 106775T) andStreptomyces triticirhizaesp.；分别提出了（NEAU-YY642T=CCTCC AA 2018092T=DSM 107172T）。
Two novelActinobacteria, designated strains NEAU-YY421Tand NEAU-YY642T, were isolated from the rhizosphere soil of wheat (Triticum aestivumL.) collected from Zhumadian, Henan Province, PR China and characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains NEAU-YY421Tand NEAU-YY642Tbelonged to the genusStreptomycesand strain NEAU-YY421Twas most closely related toStreptomyces fumanusCGMCC 4.1732T(97.9 % sequence similarity) andStreptomyces naganishiiDSM 40282T(97.8 %), and that of strain NEAU-YY642TtoStreptomyces zhaozhouensisLZS-5T(98.0 %) andStreptomyces sediYIM 65188T(97.5 %). The cell walls of the two strains containedll-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were glucose and ribose. Multilocus sequence analysis using the concatenated sequences of theatpD,gyrB,recA,rpoBandtrpBgenes showed that the two strains formed separate branches in the genusStreptomyces. Moreover, a combination of DNA–DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Therefore, strains NEAU-YY421Tand NEAU-YY642Tbelong to two novel species in the genusStreptomyces, for which the namesStreptomyces triticagrisp. nov. (NEAU-YY421T=CGMCC 4.7476T=DSM 106775T) andStreptomyces triticirhizaesp. nov. (NEAU-YY642T=CCTCC AA 2018092T=DSM 107172T) are proposed, respectively.