Extraction and Quantification of Cyclic Di-GMP from P. aeruginosa.
Extraction and Quantification of Cyclic Di-GMP from P. aeruginosa.
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DOI：
10.21769/bioprotoc.828
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发表时间：
2013-07
期刊：
影响因子：
0.8
通讯作者：
Ankita Roy;O. Petrova;K. Sauer
中科院分区：
文献类型：
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作者：
Ankita Roy;O. Petrova;K. Sauer
环二gmp （c-di-GMP）是一种重要的细胞内信号分子，在多种微生物中控制浮游（自由生活）和无根生活方式之间的转变、生物膜的形成和毒力。下面的程序描述了铜绿假单胞菌样品中c-二gmp的提取和定量。我们已尽一切努力使该方案尽可能具有通用性，以使该程序适用于各种细菌中c-二gmp水平的分析。然而，可能需要一些修改来分析其他细菌的c-二gmp水平。
Cyclic di-GMP (c-di-GMP) has emerged as an important intracellular signaling molecule, controlling the transitions between planktonic (free-living) and sessile lifestyles, biofilm formation, and virulence in a wide variety of microorganisms. The following protocol describes the extraction and quantification of c-di-GMP from Pseudomonas aeruginosa samples. We have made every effort to keep the protocol as general as possible to enable the procedure to be applicable for the analysis of c-di-GMP levels in various bacterial species. However, some modifications may be required for the analysis of c-di-GMP levels in other bacterial species.