Interferometric Reflectance Imaging Sensor (IRIS) for Molecular Kinetics with a Low-Cost, Disposable Fluidic Cartridge
Interferometric Reflectance Imaging Sensor (IRIS) for Molecular Kinetics with a Low-Cost, Disposable Fluidic Cartridge
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DOI：
10.1007/978-1-4939-9616-2_2
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Unlu, M. Selim
中科院分区：
文献类型：
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作者：
Needham, James W.;Unlu, Nese Lortlar;Unlu, M. Selim
动力学信息和合适的结合对的确定是在免疫测定、诊断和治疗中适当优化和选择配体的基础。然而，以多路复用和廉价的形式估计这些参数的能力仍然很困难，并且常常需要对配体进行修饰。在这里，我们详细介绍了使用干涉反射成像传感器（IRIS）同时评估数百种未标记配体所需的方法和材料。集成在传感器顶部的低成本流体盒大大简化了试剂处理。
YThe determination of kinetic information and appropriate binding pairs is fundamental to the proper optimization and selection of ligands used in immunoassays, diagnostics, and therapeutics. However, the ability to estimate such parameters in a multiplexed and inexpensive format remains difficult and modification of the ligand is often necessary. Here, we detail the methods and materials necessary to evaluate hundreds of unlabeled ligands simultaneously using the interferometric reflectance imaging sensor (IRIS). The incorporation of a low-cost fluidic cartridge that integrates on the top of the sensor simplifies reagent handling considerably.