Candida tropicalis affects the virulence profile of Candida albicans: an in vitro and in vivo study
Candida tropicalis affects the virulence profile of Candida albicans: an in vitro and in vivo study
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DOI：
10.1093/femspd/fty014
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发表时间：
2018-03-01
影响因子：
3.3
通讯作者：
Cardoso Jorge, Antonio Olavo
中科院分区：
文献类型：
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作者：
de Barros, Patricia Pimentel;Rossoni, Rodnei Dennis;Cardoso Jorge, Antonio Olavo
白色念珠菌（Candida albicans）和热带念珠菌（Candida tropicalis）是存在于约50%-70%健康个体口腔中的口腔微生物。然而，这些微生物可以通过环境的变化或宿主免疫系统的减弱而成为致病性的。因此，本研究的目的是评估念珠菌属的物种之间的相互作用，在生物膜的形成，表达，基因表达和毒力的大蜡螟。共孵育C.白色念珠菌（C. albicans）; 48小时后，通过降低活细胞计数、代谢活性和菌丝生长，热带假丝酵母细胞导致生物膜形成的显著减少。梭实时荧光定量PCR检测BCR 1、CPH 1、EFG 1、UME 6、HWP 1、ALS 3、SAP 5和PLB 2基因表达，发现大部分基因表达下调。在体内试验中，幼虫接受C.白色念珠菌和C.与对照组相比，热带假丝酵母的存活率显著增加。白色念珠菌（P = 0.0001）与体外结果一致。结论是C. tropicalis定殖与C.白色念珠菌，表明这两个物种之间的拮抗关系。因此，C. tropicalis通过还原C.白色念珠菌的毒力谱可能限制这种致病真菌引起感染的能力。
Candida albicans and Candida tropicalis are commensal microorganisms occurring in the oral cavity of approximately 50%-70% of healthy individuals. However, these microbes can become pathogenic through changes in the environment or weakened host immune system. Thus, the aim of this investigation was to evaluate the interaction between species of the genus Candida in the biofilm formation, filamentation, gene expression and virulence in Galleria mellonella. Coincubation of C. albicans with C. tropicalis cells after 48 h resulted in significant reduction of biofilm formation by decreasing viable cell counts, metabolic activity and hyphal growth. The C. albicans genes (BCR1, CPH1, EFG1, UME6, HWP1, ALS3, SAP5 and PLB2) were quantified by quantitative real-time polymerase chain reaction and most of genes were downregulated. Regarding in vivo assay, the groups that the larvae received C. albicans and C. tropicalis had a significant survival increase compared to the control group of C. albicans (P = 0.0001) in agreement with the in vitro results. In conclusion, C. tropicalis colonization was associated with a decrease in the growth of C. albicans, suggesting an antagonistic relation between these two species. Therefore, C. tropicalis by reducing C. albicans virulence profile may limit the ability of this pathogenic fungus to cause infection.