Enhanced bone apposition around biofunctionalized sandblasted and acid-etched titanium implant surfaces - A histomorphometric study in miniature pigs
Enhanced bone apposition around biofunctionalized sandblasted and acid-etched titanium implant surfaces - A histomorphometric study in miniature pigs
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DOI：
10.1111/j.1600-0501.2005.01222.x
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发表时间：
2006-06-01
影响因子：
4.3
通讯作者：
Buser, D
中科院分区：
文献类型：
--
作者：
Germanier, Y;Tosatti, S;Buser, D
与机加工钛表面相比，牙科种植体的微粗糙钛（Ti）表面显示出更快和更大的骨沉积。然而，通过用共聚物的单分子吸附层-即，聚（L-赖氨酸）-接枝-聚（乙二醇）（PLL-g-PEG）及其衍生物（PLL-g-PEG/PEG-肽）从未被研究过。本研究的目的是检查早期骨附着到改性喷砂和酸蚀刻（SLA）表面涂有Arg-Gly-Asp（RGD）-肽改性聚合物（PLL-g-PEG/PEG-RGD）在小型猪的上颌骨，并将其与标准SLA表面进行比较。试验和对照植入物具有相同的微粗糙形貌（SLA），但其表面化学（聚合物涂层）不同。对以下表面进行组织形态计量学检查：（i）对照-无涂层的SLA;（ii）（PLL-g-PEG）;（iii）（PLL-g-PEG/PEG-RDG）（RDG，Arg-Asp-Gly）;和（iv）（PLL-g-PEG/PEG-RGD）。在2周时，与对照组相比，RGD涂层种植体显示出显著更高的骨-种植体接触百分比（61.68% vs. 43.62%; P < 0.001）。可以得出结论，（PLL-g-PEG/PEG-RGD）涂层可以在骨再生的早期阶段促进增强的骨沉积。
Microrough titanium (Ti) surfaces of dental implants have demonstrated more rapid and greater bone apposition when compared with machined Ti surfaces. However, further enhancement of osteoblastic activity and bone apposition by bio-functionalizing the implant surface with a monomolecular adsorbed layer of a co-polymer - i.e., poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its derivatives (PLL-g-PEG/PEG-peptide) - has never been investigated. The aim of the present study was to examine early bone apposition to a modified sandblasted and acid-etched (SLA) surface coated with an Arg-Gly-Asp (RGD)-peptide-modified polymer (PLL-g-PEG/PEG-RGD) in the maxillae of miniature pigs, and to compare it with the standard SLA surface. Test and control implants had the same microrough topography (SLA), but differed in their surface chemistry (polymer coatings). The following surfaces were examined histomorphometrically: (i) control - SLA without coating; (ii) (PLL-g-PEG); (iii) (PLL-g-PEG/PEG-RDG) (RDG, Arg-Asp-Gly); and (iv) (PLL-g-PEG/PEG-RGD). At 2 weeks, RGD-coated implants demonstrated significantly higher percentages of bone-to-implant contact as compared with controls (61.68% vs. 43.62%; P < 0.001). It can be concluded that the (PLL-g-PEG/PEG-RGD) coatings may promote enhanced bone apposition during the early stages of bone regeneration.