Highly sensitive surface plasmon resonance immunosensor for parts-per-trillion level detection of 2,4,6-trinitrophenol
Highly sensitive surface plasmon resonance immunosensor for parts-per-trillion level detection of 2,4,6-trinitrophenol
复制标题
DOI：
10.1016/j.snb.2004.02.010
复制
发表时间：
2004-05-15
影响因子：
8.4
通讯作者：
Miura, N
中科院分区：
文献类型：
--
作者：
Shankaran, DR;Gobi, KV;Miura, N
利用三硝基苯酚-牛血清白蛋白（TNP- bsa）偶联物和抗TNP抗体的间接竞争免疫反应原理，建立了一种新的表面等离子体共振（SPR）免疫传感器，用于检测2,4,6-三硝基酚（TNP）。通过物理吸附将TNP-BSA偶联物固定在SPR芯片上。溶液中的TNP与固定化TNP- bsa偶联物竞争与抗TNP抗体结合，抑制TNP- bsa偶联物与抗TNP抗体的免疫反应。利用抑制作用对TNP浓度的依赖关系定量测定TNP。使用胃蛋白酶溶液可以实现多种分析的传感表面再生。该传感器对10 ~ 100 ppb浓度范围内的TNP检测具有良好的灵敏度，具有良好的分析特性，可扩展到硝基芳香族爆炸物的检测，应用于地雷的现场检测。(C) 2004 Elsevier B.V.版权所有
A new surface plasmon resonance (SPR) immunosensor has been demonstrated for the determination of 2,4,6-trinitrophenol (TNP) based on the principle of indirect competitive immunoreaction using trinitrophenol-bovine serum albumin (TNP-BSA) conjugate and anti-TNP antibody. TNP-BSA conjugate was immobilized on a SPR chip by physical adsorption. TNP in solution competes with the immobilized TNP-BSA conjugate for binding with anti-TNP antibody, which inhibit the immunoreaction between TNP-BSA conjugate and anti-TNP antibody. The dependence of the inhibition to the concentration of TNP was utilized for quantification of TNP. Regeneration of the sensing surface for multiple analyses can be effected using pepsin solution. The sensor exhibited excellent sensitivity for the detection of TNP in a wide concentration range from 10 ppt to 100 ppb and has promising analytical characteristics, which can be extended to the determination of nitroaromatic explosive compounds for application to on-site detection of landmines. (C) 2004 Elsevier B.V. All rights reserved.