Saliva is a reliable tool to detect SARS-CoV-2
Saliva is a reliable tool to detect SARS-CoV-2
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DOI：
10.1016/j.jinf.2020.04.005
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发表时间：
2020-07-01
影响因子：
28.2
通讯作者：
Baj, Andreina
中科院分区：
文献类型：
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作者：
Azzi, Lorenzo;Carcano, Giulio;Baj, Andreina
目的：对新冠肺炎患者唾液标本进行检测，并与临床及实验室资料进行比较。方法：采用逆转录-聚合酶链式反应技术对25例新冠肺炎患者唾液标本进行检测。收集了以下数据：年龄、性别、合并症、药物。在采集唾液拭子的同一天记录乳酸脱氢酶(LDH)和超敏反应C蛋白(UsRCP)。将唾液阳性的患病率以及临床资料与周期阈值之间的相关性作为病毒载量的半定量指标。结果：25名受试者进入本研究，男17人，女8人。平均年龄为61.5+/-11.2岁。65.22%的患者存在心血管和/或代谢紊乱。所有样本的SARS-CoV-2检测均呈阳性，而乳酸脱氢酶与Ct值呈负相关。2例患者咽拭子或呼吸道拭子检测结果均在同一天呈阳性。结论：唾液是检测SARS-CoV-2的可靠工具。唾液在新冠肺炎诊断中的作用不能仅限于对病毒的定性检测，它还可能提供有关疾病临床演变的信息。(C)2020年提交人(S)。由爱思唯尔有限公司代表英国感染协会出版。
Objectives: This study analyzed salivary samples of COVID-19 patients and compared the results with their clinical and laboratory data.Methods: Salivary samples of 25 COVID-19 patients were analyzed by rRT-PCR. The following data were collected: age, sex, comorbidities, drugs. Lactate dehydrogenase (LDH) and ultrasensitive reactive C protein (usRCP) values were registered on the same day when a salivary swab was collected. Prevalence of positivity in saliva and association between clinical data and the cycle threshold as a semiquantitative indicator of viral load were considered.Results: Twenty-five subjects were recruited into this study, 17 males and 8 females. The mean age was 61.5 +/- 11.2 years. Cardiovascular and/or dysmetabolic disorders were observed in 65.22% of cases. All the samples tested positive for the presence of SARS-CoV-2, while there was an inverse association between LDH and Ct values. Two patients showed positive salivary results on the same days when their pharyngeal or respiratory swabs showed conversion.Conclusions: Saliva is a reliable tool to detect SARS-CoV-2. The role of saliva in COVID-19 diagnosis could not be limited to a qualitative detection of the virus, but it may also provide information about the clinical evolution of the disease. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of The British Infection Association.