Yeast homologue of neuronal frequenin is a regulator of phosphatidylinositol-4-OH kinase
Yeast homologue of neuronal frequenin is a regulator of phosphatidylinositol-4-OH kinase
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DOI：
10.1038/12058
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发表时间：
1999-08-01
影响因子：
21.3
通讯作者：
Thorner, J
中科院分区：
文献类型：
--
作者：
Hendricks, KB;Wang, BQ;Thorner, J
在后生动物中，某些钙调素相关的钙结合蛋白（recoverins，neurocalcins和frequenins）在兴奋细胞中的水平最高，但它们的生理作用在很大程度上是未知的。在这里，我们表明，酿酒酵母含有frequenin同系物，Frq 1，它的目标是Pik 1，磷脂酰肌醇-4-OH激酶。Frq 1结合到Pik 1的催化结构域之外的Pik 1中的保守序列基序，并在体外刺激其活性。N-肉豆蔻酰化的Frq 1也可能有助于Pik 1定位。
In metazoans, certain calmodulin-related calcium-binding proteins (recoverins, neurocalcins and frequenins) are found at highest levels in excitable cells, but their physiological roles are largely uncharacterized. Here we show that Saccharomyces cerevisiae contains a frequenin homologue, Frq1, and that its target is Pik1, a phosphatidylinositol-4-OH kinase. Frq1 binds to a conserved sequence motif in Pik1 outside Pik1's catalytic domain and stimulates its activity in vitro. N-myristoylated Frq1 may also assist in Pik1 localization.