Genetic variants in glucocorticoid and mineralocorticoid receptors are associated with concentrations of plasma cortisol, muscle glycogen content, and meat quality traits in male Nellore cattle
Genetic variants in glucocorticoid and mineralocorticoid receptors are associated with concentrations of plasma cortisol, muscle glycogen content, and meat quality traits in male Nellore cattle
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DOI：
10.1016/j.domaniend.2014.12.004
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发表时间：
2015-04-01
影响因子：
2.1
通讯作者：
Balieiro, J. C. C.
中科院分区：
文献类型：
--
作者：
Poleti, M. D.;DeRijk, R. H.;Balieiro, J. C. C.
糖皮质激素受体(GR)和盐皮质激素受体(MR)是调节下丘脑-垂体-肾上腺神经内分泌轴的关键成分，协调对应激剂的生理反应以重建内环境平衡。GR(NR3C1)和MR(NR3C2)基因的遗传变异可以解释动物为适应挑战而发生的变化，从而解释它们对生产性状的影响。本研究旨在确定牛NR3C1和NR3C2基因的单核苷酸多态(SNPs)，并探讨其与肉牛生产相关性状的关系。收集了241头尼罗尔公牛(未去势119头，手术去势122头)的基因型和表型，平均年龄24+/-1.2mo，活重508+/-39 kg。分别于死后第1、7、14天测定血浆促肾上腺皮质激素(ACTH)和皮质醇浓度、肌肉糖原和乳酸含量，以及pH、颜色、蒸煮损失和胸长肌剪切力。共检测到5个SNP，其中2个位于NR3C1基因，3个位于NR3C2基因。NR3C1g.3293A和Gt；G SNP与死后血浆皮质醇浓度呈正相关(P=0.0008)。NR3C2_1g.115T>；C和NR3C2_2 g.570T>；C与肌肉糖原含量(P=0.0306和P=0.0158)、死后血浆促肾上腺皮质激素浓度(P=0.0118和P=0.095)、1d龄牛排蒸煮损失(P=0.0398和P=0.0423)相关。单倍型分析显示GR单倍型与死后血浆皮质醇浓度和MR单倍型与肉色、烹调损失、肌肉糖原含量和血浆ACTH浓度相关。本研究中观察到的相关性表明，GR和MR基因的SNPs与牛下丘脑-垂体-肾上腺轴活动和代谢谱的变化有关，从而导致肉质性状的个体差异。(C)2015 Elsevier Inc.保留所有权利。
The glucocorticoid receptor (GR) and, mineralocorticoid receptor (MR) are key components in the regulation of the hypothalamic-pituitary-adrenal neuroendocrine axis and coordinate the physiological response to stress agents to reestablish homeostasis. Genetic variations of GR (NR3C1) and MR (NR3C2) genes could explain the alterations in animals to adapt to challenges, and therefore, their influence on production traits. The present study aimed to identify single-nucleotide polymorphisms (SNPs) in the bovine NR3C1 and NR3C2 genes and explore their associations to relevant traits of beef cattle production. Genotypes and phenotypes were collected from 241 male Nellore cattle (119 noncastrated and 122 castrated surgically) with an average of 24 +/- 1.2 mo of age and live weight of 508 +/- 39 kg. The traits evaluated were concentrations of plasma adrenocorticotropic hormone (ACTH) and cortisol, muscle glycogen and lactate content, and pH, color, cooking loss, and shear force of longissimus thoracis measured on the 1st, 7th, and 14th days postmortem. Five SNPs were identified, 2 in the NR3C1 gene and 3 in the NR3C2 gene. There was an associative relationship between the SNP NR3C1_1 g.3293A>G and postmortem plasma concentration of cortisol (P = 0.0008). The SNPs NR3C2_1 g.115T>C and NR3C2_2 g.570T>C were associated with muscle glycogen content (P = 0.0306 and P = 0.0158), postmortem plasma concentration of ACTH (P = 0.0118 and P = 0.0.095), and cooking loss of the steak aged 1 d (P = 0.0398 and P = 0.0423). Haplotype analysis showed associations of GR haplotypes with postmortem plasma concentrations of cortisol and MR haplotypes with meat color, cooking losses, muscle glycogen content, and plasma concentrations of ACTH. The associations observed in the present study show that SNPs in GR and MR genes are related with changes of hypothalamic-pituitary-adrenal axis activity and metabolic profile in cattle, leading to individual variation in meat quality traits. (C) 2015 Elsevier Inc. All rights reserved.