Plasma Exosomal miR-422a and miR-125b-2-3p Serve as Biomarkers for Ischemic Stroke
Plasma Exosomal miR-422a and miR-125b-2-3p Serve as Biomarkers for Ischemic Stroke
复制标题
血浆外泌体 miR-422a 和 miR-125b-2-3p 作为缺血性中风的生物标志物。
DOI：
10.2174/1567202614666171005153434
复制
发表时间：
2017-01-01
影响因子：
2.1
通讯作者：
Li, Jin-Pin
中科院分区：
文献类型：
--
作者：
Li, Dong-Bin;Liu, Jing-Li;Li, Jin-Pin
背景：微小RNA（miRNA）水平与疾病病理生理学相关，并且在血浆外泌体中较高。血浆外泌体miRNAs在某些疾病中作为潜在的治疗靶点和诊断生物标志物，但很少有研究在缺血性卒中（IS）中对其进行研究。因此，我们探讨了血浆外泌体miR-422 a和miR-125 b-2- 3 p在不同IS阶段（急性期和亚急性期）的潜在预测价值。患者分为两组：急性期（第1-3天）27例患者和亚急性期（第4-14天）28例患者。通过定量实时聚合酶链反应（qRT-PCR）检测miR-422 a和miR-125 b-2- 3 p的血浆外泌体水平。结果：血浆外泌体miR-422 a和miR-125 b-2- 3 p表达水平在亚急性期组显著降低（P
Background: MircroRNA (MiRNA) levels are associated with disease pathophysiology and are high in plasma exosomes. Plasma exosomal miRNAs serve as potential therapeutic targets and diagnosis biomarkers in some diseases but few studies have examined them in Ischemic Stroke (IS). Therefore, we explored the potential predictive value of plasma exosomal miR-422a and miR-125b-2-3p in different IS phases (acute and subacute phases).Methods: Fifty-five IS patients and 25 age and sex matched healthy controls were recruited. Patients were classified into two groups: 27 patients in acute phase (days 1-3) and 28 patients in subacute phase (days 4-14). The plasma exosomal levels of miR-422a and miR-125b-2-3p were examined via quantitative real-time polymerase chain reaction (qRT-PCR). The Areas Under the Curve (AUC) of the Receiver Operating Characteristic (ROC) curve were constructed to evaluate the diagnostic accuracy of these miRNAs in IS.Results: The expression levels of plasma exosomal miR-422a and miR-125b-2-3p were significantly decreased in the subacute phase group (P