THE DEPENDENCY OF NUCLEAR DIVISION ON VOLUME IN THE DIMORPHIC YEAST CANDIDA-ALBICANS
THE DEPENDENCY OF NUCLEAR DIVISION ON VOLUME IN THE DIMORPHIC YEAST CANDIDA-ALBICANS
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DOI：
10.1016/0014-4827(81)90356-6
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发表时间：
1981-01-01
影响因子：
3.7
通讯作者：
BRUMMEL, M
中科院分区：
文献类型：
--
作者：
SOLL, DR;BEDELL, G;BRUMMEL, M
当二型酵母C.白念珠菌被诱导同步形成菌丝体，90%以上的细胞进行核分裂。当诱导静止期细胞同步形成芽时，即使全部形成芽，也只有不到一半的细胞进行核分裂。大多数不进行核分裂的细胞形成体积低于阈值的芽，而大多数进行核分裂的细胞形成体积高于该阈值的芽。研究了形成小芽的细胞没有达到特定质量阈值的可能性。在同步芽和同步菌丝体形成过程中，对细胞培养物的DNA复制、干重和蛋白质含量进行了检查。超过一半的萌芽群体缺乏核分裂是由于子细胞体积小或表面积小，而不是因为它们未能达到质量阈值或复制它们的DNA。核分裂对子细胞体积的依赖性进行了讨论。
When stationary phase cells of the dimorphic yeast C. albicans are induced to synchronously form mycelia, over 90% of the cells undergo nuclear division. When stationary phase cells are induced to synchronously form buds, less than half undergo nuclear division even though all form buds. The majority of cells which do not undergo nuclear division form buds with volumes below a threshold value and the majority of cells which do undergo nuclear division form buds with volumes above this threshold value. The possibilities that cells which form small buds do not attain a particular mass threshold were investigated. Cell cultures were examined for DNA replication, dry weight and protein content during synchronous bud and during synchronous mycelium formation. The lack of nuclear division in over half of a budding population is due to low daughter cell volumes or to low surface areas and not to their failure to attain a mass threshold or to replicate their DNA. The dependency of nuclear division on daughter cell volume is discussed.