Quantitative analyses of expression of GDNF and neurotrophins during postnatal development in rat skeletal muscles
Quantitative analyses of expression of GDNF and neurotrophins during postnatal development in rat skeletal muscles
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DOI：
10.1016/s0168-0102(03)00010-5
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发表时间：
2003-04-01
影响因子：
2.9
通讯作者：
Suzuki, H
中科院分区：
文献类型：
--
作者：
Nagano, M;Suzuki, H
神经营养因子被认为在神经肌肉系统的形成和维持中起关键作用。为了了解这些因子在出生后肌肉中的精确表达水平，我们建立了竞争性RT-PCR和双位点酶免疫分析法，定量测定了大鼠腓肠肌和比目鱼肌中神经营养因子在出生后发育过程中的表达。腓肠肌和比目鱼肌中胶质细胞源性神经营养因子（GDNF）的mRNA在出生时的神经营养因子中表达量最高，在前3个月内急剧下降，而GDNF蛋白在3个月龄时基本上存在。腓肠肌中神经营养素3和脑源性神经营养因子的mRNA和蛋白表达在生后一直保持恒定。相反，神经营养素-4的mRNA在前2周增加。在比目鱼肌中，所有的神经营养因子蛋白质随着年龄的增加的第一个月，与它们在腓肠肌中的表达形成对比。本研究结果表明，GDNF在出生后是组成性供应于神经肌肉接头（NMJ）。发育和进入成年期，表明其在维持NMJ中的重要性。其他神经营养因子的表达在发育过程中也可能根据其自身在神经肌肉回路中的作用独立调节。(C)2003年Elsevier Science爱尔兰有限公司和日本神经科学学会。All rights reserved.
Neurotrophic factors are thought to be critically involved in formation and maintenance of the neuromuscular system. To know precise expression levels of these factors in the muscles during the postnatal period, we developed competitive RT-PCR and two-site enzyme immunoassay and quantitatively measured neurotrophic factors in the rat gastrocnemius and solcus muscles during the postnatal development. mRNAs of glial cell line-derived neurotrophic factor (GDNF) in the gastrocnemius and the soleus muscles were expressed in the highest amount among the neurotrophic factors at birth and dramatically decreased in the first 3 months, while GDNF proteins substantially existed at 3 months of age. Neurotrophin-3 and brain-derived neurotrophic factor in the gastrocnemius muscle kept constant expression in mRNA and protein during the postnatal period. In contrast, mRNA of neurotrophin-4 increased in the first 2 weeks. In the soleus muscles all the neurotrophic factor proteins increased with age for the first month, contrasting with their expressions in the gastrocnemius. The present results showed that GDNF is;, constitutively supplied to the neuromuscular junction (NMJ) during postnatal. development and into adulthood, suggesting its importance in maintenance of the NMJ. Expression of other neurotrophins was also regulated independently during development possibly according to their own roles in the neuromuscular circuit. (C) 2003 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.