A fluorimetric and circular dichroism study of hemoglobin - Effect of pH and anionic amphiphiles
A fluorimetric and circular dichroism study of hemoglobin - Effect of pH and anionic amphiphiles
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DOI：
10.1016/j.jcis.2005.08.047
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发表时间：
2006-04-01
影响因子：
9.9
通讯作者：
Girigoswami, A
中科院分区：
文献类型：
--
作者：
De, S;Girigoswami, A
本工作主要采用荧光法对牛血红蛋白（Hb）进行了研究。已经发现pH对Hb结构产生深远的影响。这已经通过荧光和圆二色性（CD）研究证实。pH引起的血红蛋白四级结构的变化间接影响其二级结构。这反过来又会影响配体结合血红蛋白在不同的pH值。两个两亲物，胆汁盐和表面活性剂的结合，进行了研究。用已知的变性剂尿素和极性探针ANS（已用作外源荧光团）研究证实了Hb的pH诱导结构修饰。(c)2005年由Elsevier Inc.出版
In this work, bovine hemoglobin (Hb) has been Studied mainly by the fluorescence method. pH has been found to exert a profound effect on Hb structure. This has been confirmed by fluorescence and circular dichroism (CD) studies. The pH-induced change in quaternary structure of Hb indirectly affects its secondary structure. This in turn affects ligand binding to Hb at various pH. The binding of two amphiphiles, a bile salt and a surfactant, have been investigated. The pH-induced structural modification of Hb has been confirmed by studies with the well-known denaturant urea and the polarity probe ANS, which has been used Lis an extrinsic fluorophore. (c) 2005 Published by Elsevier Inc.