Evidence that stimulation of thyrotropin and prolactin secretion by thyrotropin-releasing hormone occur via different calcium-mediated mechanisms: studies with verapamil.
Evidence that stimulation of thyrotropin and prolactin secretion by thyrotropin-releasing hormone occur via different calcium-mediated mechanisms: studies with verapamil.
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促甲状腺素释放激素刺激促甲状腺素和催乳素分泌的证据是通过不同的钙介导机制发生的：维拉帕米的研究。
DOI：
10.1210/endo-110-3-901
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
M. Gershengorn
中科院分区：
文献类型：
--
作者：
E. Geras;M. Rebecchi;M. Gershengorn
TRH刺激TSH和PRL分泌需要Ca 2+。我们确定了细胞外Ca 2+的流入是否参与TtT细胞（短期培养的小鼠垂体瘤细胞产生TSH），GH 3细胞（产生PRL的克隆大鼠垂体瘤细胞）和正常大鼠体外垂体前叶细胞中TRH的作用。TRH使TtT细胞摄取~（45）Ca ~（2+）增加40%，但对GH 3细胞摄取~（45）Ca ~（2+）无影响。维拉帕米完全抑制TtT和GH 3细胞对50 mM K+去极化引起的~（45）Ca ~（2+）的摄取，从而阻断细胞外Ca ~（2+）内流。维拉帕米（50 μ M）可阻断TRH引起的TtT细胞45 Ca ~（2+）摄取增加。维拉帕米对TRH刺激的GH 3细胞45 Ca ~（2+）外排和PRL释放无影响，但可部分抑制TtT细胞45 Ca ~（2+）外排和TSH释放。在静态孵育期间，TRH刺激的GH 3细胞的PRL释放同样不受维拉帕米的影响（7.2 ± 1.1 ng/ml vs. 6.2 ± 1.1 ng/ml）。
TRH stimulation of TSH and PRL secretion requires Ca2+. We determined whether influx of extracellular Ca2+ was involved in TRH action in TtT cells (mouse pituitary tumor cells in short term culture that produce TSH), in GH3 cells (clonal rat pituitary tumor cells that produce PRL), and in anterior pituitary cells in vitro derived from normal rats. TRH increased 45Ca2+ uptake by TtT cells by 40% but had no effect on 45Ca2+ uptake by GH3 cells. Verapamil, which totally inhibited 45Ca2+ uptake by TtT and GH3 cells induced by depolarization with 50 mM K+, was employed to block influx of extracellular Ca2+. Verapamil (50 JUM) abolished the increment in 45Ca2+ uptake by TtT cells caused by TRH. Verapamil had no effect on TRH-stimulated 45Ca2+ efflux and PRL release from GH3 cells but partially inhibited TRH-stimulated 45Ca2+ efflux and TSH release from TtT cells during perifusion. TRH-stimulated PRL release from GH3 cells was likewise not affected by verapamil during static incubation (7.2 ± 1.1 ng/ml vs. 6.2 ±...
三氟拉嗪、D600 和苯妥英对去极化和促甲状腺素释放激素诱导的大鼠垂体组织促甲状腺素释放的影响。
影响因子：
4.8
作者：
Fleckman,A;Erlichman,J;Schubart,UK;Fleischer,N
通讯作者：
Fleischer,N
证据表明 Ca 在 3,5,3-三碘甲状腺原氨酸抑制促甲状腺素释放激素诱导的体外大鼠垂体前叶促甲状腺素分泌中可能发挥作用。
影响因子：
4.8
作者：
Schrey,MP;Larsen,PR
通讯作者：
Larsen,PR