An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires
An Escherichia coli Chassis for Production of Electrically Conductive Protein Nanowires
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DOI：
10.1021/acssynbio.9b00506
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发表时间：
2020-03-20
影响因子：
4.7
通讯作者：
Lovley, Derek R.
中科院分区：
文献类型：
--
作者：
Ueki, Toshiyuki;Walker, David J. F.;Lovley, Derek R.
Geophyssulfreducens的基于菌毛蛋白的导电蛋白质纳米线（e-PN）是一种革命性的电子材料。它们为电子传感应用提供了新的选择，并具有从大气湿度中获取电能的卓越能力。然而，技术上的限制限制了G. sukurreducens。因此，我们设计了一个大肠杆菌菌株，通过引入一个含有一个诱导操纵子的质粒来表达e-PNs。大肠杆菌IV型药丸生物发生机制的基因和设计用于产生可组装成e-PN的肽单体的合成基因。用E coil表达的e-PNs经简单过滤法收获后，其直径和电导与G.硫还原菌这些结果与E.大肠杆菌进行大量培养，广泛的E.用于遗传操作的线圈工具箱，大大扩展了大规模制造新型e-PN的机会。
Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs) are a revolutionary electronic material. They offer novel options for electronic sensing applications and have the remarkable ability to harvest electrical energy from atmospheric humidity. However, technical constraints limit mass cultivation and genetic manipulation of G. sukurreducens. Therefore, we designed a strain of Escherichia coil to express e-PNs by introducing a plasmid that contained an inducible operon with E. coli genes for type IV pill biogenesis machinery and a synthetic gene designed to yield a peptide monomer that could be assembled into e-PNs. The e-PNs expressed in E coil and harvested with a simple filtration method had the same diameter (3 nm) and conductance as e-PNs expressed in G. sulfurreducens. These results, coupled with the robustness of E. coli for mass cultivation and the extensive E. coil toolbox for genetic manipulation, greatly expand the opportunities for large-scale fabrication of novel e-PNs.