Periadolescent nicotine administration produces enduring changes in dendritic morphology of medium spiny neurons from nucleus accumbens
Periadolescent nicotine administration produces enduring changes in dendritic morphology of medium spiny neurons from nucleus accumbens
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DOI：
10.1016/j.neulet.2005.05.041
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发表时间：
2005-09-09
影响因子：
2.5
通讯作者：
Smith, RF
中科院分区：
文献类型：
--
作者：
McDonald, CG;Dailey, VK;Smith, RF
本研究的目的是探讨青少年期尼古丁暴露如何影响树突状形态的中型多刺神经元从核壳。从出生后第22天（p22）至p69，长期给予雄性Long-Evans hooded大鼠尼古丁或生理盐水。尼古丁和生理盐水通过皮下植入的渗透泵给药。在p144，尼古丁给药结束后75天，处理大脑进行Golgi-Cox染色。数字化重建来自核壳的中等多刺神经元。结果发现，尼古丁处理的动物的神经元具有显着更长的树突和更多的树突节比对照组动物。一个分支顺序分析表明，树突长度和段数的差异是最明显的第三和第四阶段。随后的行为实验表明，观察到的解剖学变化与持久的心理差异有关。这些研究结果表明，青少年期暴露于尼古丁可能导致持久的结构变化的核壳，并与行为数据表明，青少年尼古丁暴露可能会导致在成年尼古丁成瘾的脆弱性是一致的。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The objective of the current study was to examine how periadolescent nicotine exposure affects dendritic morphology of medium spiny neurons from the nucleus accumbens shell. Male Long-Evans hooded rats were chronically administered nicotine or saline for a period extending from postnatal day 22 (p22) to p69. Nicotine and saline administration was via subcutaneously implanted osmotic pumps. At p144, 75 days after conclusion of nicotine administration, brains were processed for Golgi-Cox staining. Medium spiny neurons from the nucleus accumbens shell were digitally reconstructed. It was found that neurons from nicotine-treated animals possessed significantly longer dendrites and a greater number of dendritic segments than control animals. A branch order analysis indicated that differences in dendritic length and segment number were most pronounced in third and fourth order segments. A subsequent behavioral experiment suggests that the observed anatomical changes are associated with enduring psychomotor differences. These findings indicate that periadolescent exposure to nicotine can result in long-lasting structural changes in the nucleus accumbens shell and are consistent with behavioral data suggesting that adolescent nicotine exposure may result in vulnerability to nicotine addiction in adulthood. (c) 2005 Elsevier Ireland Ltd. All rights reserved.