A System for Automated, Noninvasive, Morphology-Based Evaluation of Induced Pluripotent Stem Cell Cultures
A System for Automated, Noninvasive, Morphology-Based Evaluation of Induced Pluripotent Stem Cell Cultures
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DOI：
10.1177/2211068214537258
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发表时间：
2014-10-01
期刊：
影响因子：
--
通讯作者：
Loewke, Kevin
中科院分区：
文献类型：
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作者：
Maddah, Mahnaz;Shoukat-Mumtaz, Uzma;Loewke, Kevin
由于近年来人类诱导多能干细胞(IPSCs)的快速采用和使用，需要新的技术来标准化IPSCs的评估，以便能够对不同实验和组的结果进行客观比较。在这篇文章中，我们提出了一个非侵入性的，全自动的，基于形态学的IPSC培养评估分析系统，它由时间推移显微镜和新的图像分析软件组成。该系统采集IPSC在培养数天内生长的微光位相对比图像，测量IPSC在整个时间内的基于几何和纹理的特征，并推导出一组六个生物相关特征来自动对细胞培养的质量进行评级。在对94种IPSC文化的研究中，我们通过将自动排名与基于延时电影视觉审查的独立专家评估进行比较，证明了该系统的准确性。据我们所知，这是使用非侵入性方法对IPSC培养质量进行全自动和客观评估的第一次演示。
Due to the rapid adoption and use of human induced pluripotent stem cells (iPSCs) in recent years, there is a need for new technologies that standardize the evaluation of iPSCs to allow the objective comparison of results across different experiments and groups. In this article, we present a noninvasive, fully automated, and analytical system for morphology-based evaluation of iPSC cultures that consists of time-lapse microscopy and novel image analysis software. The presented system acquires low-light phase-contrast images of iPSC growth collected during a period of several days in culture, measures geometrical-and texture-based features of iPSC colonies throughout time, and derives a set of six biologically relevant features to automatically rank the quality of the cell culture. In a study of 94 iPSC cultures, we demonstrated the accuracy of the system by comparing the automated ranking with an independent expert evaluation based on visual review of the time-lapse movies. To our knowledge, this is the first demonstration of a fully automated and objective assessment of iPSC culture quality using noninvasive methods.