MapZ Forms a Stable Ring Structure That Acts As a Nanotrack for FtsZ Treadmilling in Streptococcus mutans
MapZ Forms a Stable Ring Structure That Acts As a Nanotrack for FtsZ Treadmilling in Streptococcus mutans
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DOI：
10.1021/acsnano.8b02469
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发表时间：
2018-06-01
期刊：
影响因子：
17.1
通讯作者：
Wei, Shicheng
中科院分区：
文献类型：
--
作者：
Li, Yongliang;Shao, Shipeng;Wei, Shicheng
细菌二元细胞分裂需要精确放置分裂机器。FtsZ是细胞分裂机制的重要组成部分，可以在细胞分裂的适当位置组装成细丝并自组织成环状结构（2，环）。MapZ是最近在链球菌科中发现的一种FtsZ调节因子，已被发现定位于中间细胞，在那里它有助于正确定位FtsZ环。然而，其机制尚不清楚。在这里，我们利用全内反射荧光显微镜、超分辨率成像和单分子跟踪，研究了MapZ控制FtsZ环位置的机制。我们的研究结果表明，FtsZ在S. mu . tan中表现出动态的跑步运动。重要的是，MapZ的耗尽导致跑步FtsZ丝的不受约束的运动和收缩FtsZ环的寿命缩短，这经常是错位的。此外，通过揭示MapZ在分裂位点形成一个固定的环状纳米结构，我们的研究表明，MapZ形成了一个稳定的环，作为一个纳米轨道来引导和限制变形杆菌中的FtsZ细丝的踩踏。
Bacterial binary cell division requires accurate placement of division machinery. FtsZ, a vital component of the division machinery, can assemble into filaments and self-organize into a ring structure (2, ring) at the appropriate site for cell division. MapZ, a recently identified FtsZ regulator in Streptococcaceae, has been found to localize at the midcell where it helps to properly position the FtsZ ring. However, its mechanism is still unclear. Here, by using total internal reflection fluorescence microscopy, super-resolution imaging, and single molecule tracking, we investigated the mechanism by which MapZ controls the position of the FtsZ ring. Our results show that FtsZ exhibits a dynamic treadmilling motion in S. mu tans. Importantly, depletion of MapZ leads to the unconstrained movement of treadmilling FtsZ filaments and a shorter lifetime of the constricting FtsZ ring, which is frequently misplaced. Furthermore, by revealing that MapZ forms an immobile ring-like nanostructure at the division site, our study suggests that MapZ forms a stable ring that acts as a nanotrack to guide and restrict treadmilling FtsZ filaments in S. mutans.