TY3 INTEGRATES WITHIN THE REGION OF RNA POLYMERASE-III TRANSCRIPTION INITIATION
TY3 INTEGRATES WITHIN THE REGION OF RNA POLYMERASE-III TRANSCRIPTION INITIATION
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DOI：
10.1101/gad.6.1.117
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发表时间：
1992-01-01
影响因子：
10.5
通讯作者：
SANDMEYER, SB
中科院分区：
文献类型：
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作者：
CHALKER, DL;SANDMEYER, SB
恢复并绘制了逆转录病毒样元件 Ty3 目标质粒中超过 190 个独立的插入片段。 Ty3 显示插入 tRNA、5S 和 U6 基因的上游，所有这些基因均由 RNA 聚合酶 III 转录。整合位点位于转录起始位置的 1-4 个核苷酸内，即使对于其中聚合酶 III 起始位点转移到全新环境的突变基因也是如此。 SUP2 tRNA 基因靶标的诱变表明整合需要功能性启动子元件，但它与靶标转录没有简单的关联。这是第一份将离散基因组功能与逆转录转座子优先插入直接联系起来的报告。
Over 190 independent insertions into target plasmids of the retrovirus-like element Ty3 were recovered and mapped. Ty3 was shown to insert upstream of tRNA, 5S, and U6 genes, all of which are transcribed by RNA polymerase III. Integration sites were within 1-4 nucleotides of the position of transcription initiation, even for one mutant gene where the polymerase III initiation site was shifted to a completely new context. Mutagenesis of a SUP2 tRNA gene target showed that integration required functional promoter elements but that it did not correlate in a simple way with target transcription. This is the first report directly linking a discrete genomic function with preferential insertion of a retrotransposon.