Tree Species Shape Soil Bacterial Community Structure and Function in Temperate Deciduous Forests
Tree Species Shape Soil Bacterial Community Structure and Function in Temperate Deciduous Forests
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DOI：
10.3389/fmicb.2019.01519
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发表时间：
2019-07-09
影响因子：
5.2
通讯作者：
Daniel, Rolf
中科院分区：
文献类型：
--
作者：
Dukunde, Amalie;Schneider, Dominik;Daniel, Rolf
基于扩增子的16 S rRNA基因和转录本的分析被用来评估树种组成对温带落叶林土壤细菌群落结构和功能的影响。在春、夏、秋3个季节分别从山毛榉、鹅耳枥、椴树和栎类的单一林分和混交林分中采集样品。土壤细菌群落在总群落（基于DNA）和潜在活性群落（基于RNA）水平上表现出相似的分类组成，在活性群落水平上存在较少的类群。根瘤菌目在总菌群和活性菌群中均占优势，其次为酸杆菌目、假单胞菌目、红单胞菌目和黄单胞菌目。总的和活跃的社区水平的细菌群落表现出显着的正相关性与树种身份（单立场），并在较小程度上与树种丰富度（混合立场）。约58%和64%的指示操作分类单位（OTU）显示出显着的关联，只有一个单一的立场，在总的和活跃的社区水平，分别表示强烈的影响，树种对土壤细菌群落组成。土壤C/N比、pH和P含量与土壤细菌群落呈显著正相关，这是林分直接和间接作用的结果。季节性是最强的驱动力预测代谢功能相关的C固定和降解，N代谢。碳和氮代谢过程在春季显著丰富，而C降解基因丰度从夏季到秋季增加，对应于增加的凋落物和分解。结果表明，在空间均质的森林土壤中，树种多样性和丰富度是土壤细菌群落结构和组成的主要驱动因素。
Amplicon-based analysis of 16S rRNA genes and transcripts was used to assess the effect of tree species composition on soil bacterial community structure and function in a temperate deciduous forest. Samples were collected from mono and mixed stands of Fagus sylvatica (beech), Carpinus betulus (hornbeam), Tilia sp. (lime), and Quercus sp. (oak) in spring, summer, and autumn. Soil bacterial community exhibited similar taxonomic composition at total (DNA-based) and potentially active community (RNA-based) level, with fewer taxa present at active community level. Members of Rhizobiales dominated at both total and active bacterial community level, followed by members of Acidobacteriales, Solibacterales, Rhodospirillales, and Xanthomonadales. Bacterial communities at total and active community level showed a significant positive correlation with tree species identity (mono stands) and to a lesser extent with tree species richness (mixed stands). Approximately 58 and 64% of indicator operational taxonomic units (OTUs) showed significant association with only one mono stand at total and active community level, respectively, indicating a strong impact of tree species on soil bacterial community composition. Soil C/N ratio, pH, and P content similarly exhibited a significant positive correlation with soil bacterial communities, which was attributed to direct and indirect effects of forest stands. Seasonality was the strongest driver of predicted metabolic functions related to C fixation and degradation, and N metabolism. Carbon and nitrogen metabolic processes were significantly abundant in spring, while C degradation gene abundances increased from summer to autumn, corresponding to increased litterfall and decomposition. The results revealed that in a spatially homogenous forest soil, tree species diversity and richness are dominant drivers of structure and composition in soil bacterial communities.