Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences
Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences
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DOI：
10.1007/s11427-008-0057-1
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发表时间：
2008-04
期刊：
影响因子：
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通讯作者：
ZiHui Li;Xian-min Feng;Siqi Lu;Fan Zhang;Feng-yun Wang;Song Huang
中科院分区：
文献类型：
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作者：
ZiHui Li;Xian-min Feng;Siqi Lu;Fan Zhang;Feng-yun Wang;Song Huang
为阐明肺孢子虫的系统发育关系和种属地位，本研究对大鼠、沙鼠和人肺孢子虫5.8SrRNA基因和内转录间隔区（ITS，1和2）进行了扩增、克隆和序列测定。与其他真菌如塔弗氏菌属和放线菌属的遗传距离矩阵表明，肺孢子虫属中包含多个种，包括沙鼠肺孢子虫。系统发育树显示，人源肺孢子虫和猴源肺孢子虫为一个类群，4种啮齿类肺孢子虫为另一个类群。在4个成员中，与鼠肺孢子虫和卡氏肺孢子虫的亲缘关系最近，与沙鼠肺孢子虫的亲缘关系最远。
To clarify the phylogenetic relationships and species status ofPneumocystis, the 5.8S rRNA gene and the internal transcribed spacers (ITS, 1 and 2) ofPneumocystisrRNA derived from rat, gerbil and human were amplified, cloned and sequenced. The genetic distance matrix of sixPneumocystisspecies compared with other fungi likeTaphrinaandSaccharomycesindicated that thePneumocystisgenus contained multiple species includingPneumocystisfrom gerbil. The phylogenetic tree also showed thatPneumocystisfrom human and monkey formed one group and four rodentPneumocystisformed another group. Among the four members,Pneumocystis wakefieldiaewas most closely related toPneumocystis murinaandPneumocystis carinii, and was least related to gerbilPneumocystis.