Polyunsaturated Fatty Acids Differentially Modulate Cell Proliferation and Endocannabinoid System in Two Human Cancer Lines
Polyunsaturated Fatty Acids Differentially Modulate Cell Proliferation and Endocannabinoid System in Two Human Cancer Lines
复制标题
DOI：
10.1016/j.arcmed.2017.01.009
复制
发表时间：
2017-01-01
影响因子：
7.7
通讯作者：
Eynard, Aldo R.
中科院分区：
文献类型：
--
作者：
Gaston, Repossi;Maria Eugenia, Pasqualini;Eynard, Aldo R.
背景和目标。有证据表明，饮食中多不饱和脂肪酸（PUFA）的数量和质量在癌症的发展中发挥作用。然而，这种相互作用所涉及的机制尚不清楚。内源性大麻素是已知具有生长调节作用的脂质代谢物。我们研究了补充PUFAs ω-6和ω-3（必需脂肪酸，EFA）、饱和和单不饱和脂肪酸（非EFA）对肿瘤细胞生长的影响及其内源性大麻素含量的改变。将人胶质母细胞瘤（T98 G）和乳腺癌（MCF 7）的细胞培养物补充50或100 mmol EFA和非EFA 72 h。MTT法测定细胞增殖，HPLC法测定花生四烯酸（AEA）含量，GLC法测定总脂肪酸含量，WB法测定CB 1受体表达，分光光度法测定FAAH活性。脂肪酸谱反映了各试验中补充的脂质的掺入。补充花生四烯酸（EFA omega-6）可增加AEA水平并抑制T98 G的生长，而棕榈酸（非EFA）可增强其增殖。在乳腺癌（MCF 7）细胞中，二十碳五烯酸（EFA omega-3）减少，油酸（非EFA）增强其增殖。CB 1在T98 G中表达较高，而FAAH活性无差异。肿瘤细胞的生长可以通过脂肪酸进行差异调节，并且至少部分地可以归因于它们作用于内源性大麻素系统的组分的能力。(c)2017年IMSS。爱思唯尔公司出版
Background and Aims. Evidence suggests that quantity and quality of dietary polyunsaturated fatty acids (PUFAs) play a role in the development of cancer. However, the mechanisms involved in this interaction(s) are not clear. Endocannabinoids are lipid metabolites known to have growth modulatory actions. We studied the effect of supplementation with PUFAs omega-6 and omega-3 (essential fatty acids, EFAs), saturated and monoun-saturated fatty acids (non-EFAs) on the growth of tumor cells and modifications in their endocannabinoid content.Methods. Cell cultures of human glioblastoma (T98G) and breast cancer (MCF7) were supplemented with 50 or 100 mmol EFAs and non-EFAs for 72 h. Cell proliferation was then determined by MTT, anandamide (AEA) levels by HPLC, total fatty acids profiles by GLC, CB1 receptor expression by WB and FAAH activity by spectrophotometric method.Results. Fatty acids profile reflected the incorporation of the lipids supplemented in each assay. Arachidonic acid (EFA omega-6) supplementation increased AEA levels and inhibited the growth of T98G, whereas palmitic acid (non-EFA) enhanced their proliferation. In breast cancer (MCF7) cells, eicosapentaenoic acid (EFA omega-3) reduced and oleic acid (non-EFA) enhanced their proliferation. CB1 expression was higher in T98G and no differences were observed in FAAH activity.Conclusions. The growth of tumor cells can be differentially modulated by fatty acids and, at least in part, can be attributed to their ability to act on the components of the endocannabinoid system. (c) 2017 IMSS. Published by Elsevier Inc.