Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
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短杆菌肽抑制人胃癌细胞增殖、细胞周期和诱导的细胞凋亡
DOI：
10.1186/s40659-019-0264-1
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发表时间：
2019-01-01
影响因子：
6.7
通讯作者：
Wang, Zishu
中科院分区：
文献类型：
--
作者：
Chen, Tingting;Wang, Yong;Wang, Zishu
陈婷婷等。革兰西丁抑制人胃癌细胞增殖、细胞周期及诱导凋亡。医学杂志。研究》(在线)。2019, vol. 52, 57。Epub 11月28 - - 2019。ISSN 0716 - 9760。http://dx。doi。org/10.1186/s40659 - 019 - 0264 - 1。背景：胃癌是世界范围内常见的高发病率、高死亡率的恶性肿瘤，严重影响人类健康。革兰霉素是一种短肽抗生素，可用于治疗细菌或真菌引起的感染。然而，革兰霉素对胃癌细胞的抗癌作用及其机制仍不清楚。结果：不同浓度的革兰霉素分别作用于胃癌细胞SGC-7901、BGC-823和正常胃粘膜细胞GES-1。CCK-8实验结果显示革兰西菌素对癌细胞的细胞毒性，细胞集落形成实验显示革兰西菌素显著抑制胃癌细胞的增殖，但对正常胃粘膜细胞影响不大。此外，伤口愈合实验显示革兰杀菌素抑制SGC-7901细胞的迁移。同时，细胞凋亡和细胞周期分析显示革兰西丁诱导细胞凋亡，并抑制G2/M细胞周期。western blot分析表明，gramicidin下调cyclinD1和Bcl-2的表达以及FoxO1的磷酸化。结论：本研究显示了革兰西丁对胃癌细胞的抗肿瘤活性，为革兰西丁应用于临床治疗胃癌提供了可能。
CHEN, Tingting et al. Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis. Biol. Res.[online]. 2019, vol. 52, 57. Epub 28-Nov-2019. ISSN 0716-9760. http://dx. doi. org/10.1186/s40659-019-0264-1.Background:Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, which seriously affects human health. Gramicidin is a short peptide antibiotic which could be used for treating infection induced by bacteria or fungi. However, the anti-cancer effect of gramicidin on gastric cancer cells and its underlying mechanism remains largely unknown.Results:Gastric cancer cells SGC-7901, BGC-823 and normal gastric mucosal cells GES-1 were treated with different concentrations of gramicidin respectively. The results of CCK-8 experiment revealed cellular toxicity of gramicidin to cancer cells while cell colony formation assay showed that gramicidin significantly inhibited the proliferation of gastric cancer cells, but had little effect on normal gastric mucosal cells. In addition, the wound healing assay showed that gramicidin inhibited the migration of SGC-7901 cell. Meanwhile, apoptosis and cell cycle analysis revealed that gramicidin induced cell apoptosis with G2/M cell cycle inhibition. Furthermore, western blot analysis demonstrated that gramicidin down-regulated the expression of cyclinD1 and Bcl-2 as well as the FoxO1 phosphorylation.Conclusions:The current study illustrated the anti-tumor activity of gramicidin on gastric cancer cells, providing a possibility for gramicidin to be applied in clinical practice for the treatment of gastric cancer.